pAAVdual-MP-135-EGFP

pAAVdual-MP-135-EGFP  is used to produce AAV-MP-135-EGFP virus using AAVnerGene’s novel AAVdual production system. In this plasmid,  the  regular single strand AAV genome  with a MP-135 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper.  AAV-MP-135-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper  (pRCap) plasmids, carrying AAV2 rep gene and different cap genes,  without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.

The MP-135 is a micro promoter derived from the promoter of human glucagon gene. Despite its small size (135 bp), it demonstrates activity comparable to the strong, ubiquitous CAG promoter across diverse cell types and tissues. MP-135 drives robust expression in both skeletal and cardiac muscle, making it particularly valuable for neuromuscular disease models and cardiac gene therapy. rAAV constructs containing MP-135 have also shown strong activity in cultured cells from all three germ-layer lineages, with reporter expression documented in human primary hepatocytes, pancreatic islets, and multiple mouse tissues in vivo, including brain and skeletal muscle. Its compact design conserves AAV packaging capacity, allowing more space for larger therapeutic payloads.

• AAV Cassette: AAV-MP-135-EGFP
• AAV Genome Size: 1868 bp
• Plasmid Size: 10301 bp
• Plasmid Backbone: pAAVdual
• AAV Genome Type: ssAAV
• ITRs:  L-ITR (119 bp) + R-ITR (130 bp)
• Promoter:MP-135
• Transgene: EGFP
• Bacterial Resistance(s) : Kana
• Storage:  -20°C for long term (> 1 year)
• Shipping: Dry ice.
• Notice to Purchaser:  MTA is required. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
• Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
• AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
• AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.