AAV Cell Lines
An Integrated Cell-Line Platform for AAV Manufacturing and Testing
From high-yield vector production to infectivity and rcAAV testing, AAVnerGene’s HEK293 cell-line portfolio provides specialized tools for developing more efficient and reproducible AAV manufacturing and analytical workflows.
| Cell Line | Primary Application | Key Advantage |
|---|---|---|
| HEK293one® | AAV production | High productivity and high full-particle ratio |
| HEK293R | TCID₅₀ / infectivity testing | Sensitive and reproducible AAV infectivity assays |
| HEK293RR | rcAAV testing | Enhanced platform for rcAAV detection |
HEK293one® is a proprietary suspension HEK293 cell line developed by AAVnerGene through selection and optimization with the AAVone® production platform. HEK293one® is designed to support robust suspension growth, efficient transient transfection, and high AAV productivity. It is particularly optimized for use with AAVone®2.1 and can also support conventional transient AAV production workflows.
Key Features
- Specifically selected and optimized for AAV vector production
- Robust high-density suspension growth
- High transfection efficiency and cell viability
- Supports production of multiple AAV serotypes and engineered capsids
- Optimized for the AAVone® single-plasmid platform
- Compatible with conventional triple-plasmid production
- Suitable for shake-flask and bioreactor scale-up
- Optimized for use with CelliMax™ Medium, PEIone™, and ENone™
- Suitable for AAV research, process development, and scalable manufacturing
Performance with AAVone®2.1
When HEK293one® is combined with the AAVone®2.1 production system and optimized process conditions, the platform can achieve:
🚀 AAV productivity exceeding 5 × 10¹² vg/mL
📦 Full-particle ratios of up to ~80%
The combination provides a high-performance cellular and production platform designed to improve AAV yield, product quality, reproducibility, and manufacturing efficiency.
HEK293R is an AAVnerGene HEK293-derived cell line developed for AAV infectivity and cell-based analytical assays.
The cell line is designed to provide a sensitive and reproducible cellular background for evaluating infectious AAV particles and can be incorporated into TCID₅₀-based AAV testing workflows.
Key Applications
- AAV TCID₅₀ assays
- Infectious AAV titer determination
- AAV infectivity testing
- Vector transduction characterization
- Cell-based AAV potency assay development
- Analytical method development and optimization
- AAV process-development support
HEK293R provides a standardized cellular platform for laboratories seeking to establish reproducible AAV infectivity assays.
HEK293RR is a specialized HEK293-derived cell line developed by AAVnerGene for replication-competent AAV (rcAAV) detection and analytical testing.
The cell line is designed to support sensitive detection of replication-competent AAV contaminants and can be incorporated into rcAAV assay development and quality-control workflows.
Key Applications
- Replication-competent AAV (rcAAV) testing
- Sensitive rcAAV detection
- AAV product characterization
- Assay development and qualification
- Process-development studies
- Research and quality-control applications
HEK293RR provides a dedicated cellular platform for evaluating rcAAV contamination in recombinant AAV preparations.