AAV Viruses

Premade AAV Viruses

Premade AAV viruses and vectors offer a convenient, ready-to-use solution for researchers conducting gene delivery studies, including gene therapy, gene editing, and gene regulation. AAVnerGene provides high-quality, U.S.-made AAV vectors, produced and quality controlled in the United States.

We offer a diverse selection of premade AAVs with various serotypes, promoters, reporters, and transgenes, enabling researchers to select the most suitable vectors for their specific applications. With access to a broad range of AAV serotypes, researchers can rapidly evaluate AAV tropism and identify optimal vectors for different cell and tissue types. Multiple promoter and reporter combinations also enable convenient assessment of gene-expression specificity, level, and timing.

AAVnerGene is also the official U.S. supplier of AAV GRAB Sensors  developed by Professor Yulong Li’s laboratory, a global leader in genetically encoded fluorescent biosensors. Through this partnership, AAVnerGene provides convenient access to a broad portfolio of validated biosensor AAV vectors for monitoring neurotransmitters, neuromodulators, ions, metabolites, and intracellular signaling in vitro and in vivo.

All AAV products are supported by AAVnerGene’s expertise in AAV vector design, production, purification, and quality control, with custom AAV production available for specialized research needs.

AAV Viruses

To ensure the highest quality and purity of its premade AAV viruses, AAVnerGene employs a range of rigorous assays to characterize AAV vectors:

  • Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
  • Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
  • Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG. 
  • SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
  • Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.

The high purity and quality of AAVnerGene’s premade AAV viruses offer significant advantages to customers, including reduced optimization efforts, lower labor requirements, and minimal expertise needed. This ensures reliable and consistent results for both in vitro and in vivo applications.

AAV Viruses with Different Promoters and Reporters

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AAV GRAB Bio'sensors

5-HT sensors

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AAV References

AAV ReferencesSerotypeGenomeProduction Plasmids
 AAV References (Full-2.8kb)AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eBFull of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kbpAAVone-AAVs-CMV-EGFP-2.8kb
 AAV References (Full-4.3kb)AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eBFull of AAV-CMV-SV40I-EGFP-WPRE-hGHpA-4.3kbpAAVone-AAVs-CMV-EGFP-4.3kb
AAV References (Truly-Empty)AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eBNomini-pHelper-AAVs
AAV References (Byproduct-Empty)AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eBPartial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kbpAAVone-AAVs-CMV-EGFP-2.8kb