AAV11 Reference-2.8kb
AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb
  1. AAV11 Reference-2.8kb
  2. AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb

AAV11 Reference-2.8kb

Price range: $399.00 through $2,799.00

The AAV11 reference-2.8kb material contains a 2.8kb AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA genome in AAV11 capsid, with a genome titer (EGFP targeting) of 1X10^13 VG/ml. This reference is produced using the AAVone system with the pAAVone-AAV11-CMV-EGFP-2.8kb plasmid.  AAV11 is relatively lesser-known serotype within the AAV family. Like other lesser-used AAV serotypes, it has been explored primarily in the context of understanding capsid diversity and tropism. AAV11 shares some similarities with other serotypes, but its tissue tropism and potential applications in gene therapy remain less characterized compared to more common serotypes such as AAV2, AAV8, and AAV9. Our data demonstrated that AAV11 exhibits high expression in the mouse lung at the RNA level and in the heart at the DNA level. 

  • AAV Serotype: AAV11
  • AAV Genome: AAV-CMV-SV40I-EGFP-WPRE-hGHpA-2.8k
  • AAV Genome Type and Size: ssAAV, 2843 bp, 
  • Promoter: CMV
  • Intron: SV40 Intron
  • Reporter: EGFP
  • Enhancer: WPRE
  • PolyA: hGHpolyA
  • AAV Genome Titer:  1X10^13 VG/ml, targeting WPRE
  • Full particle ratio:  >85% by mass photometry
  • Producer Plasmid: pAAVone-AAV11-CMV-EGFP-2.8kb
  • Producer Cell line: Suspension HEK 293T
  • Purification: AAVx followed by one round of CsCl ultracentrifuge.
  • Endotoxin: <1.0 EU/1X10^13 VG
  • Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
  • Storage:  -80°C for long term (> 1 year).
  • Shipping: Dry ice.
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SKU: DJ001001-AAV11 Categories: , , ,

AAV References at AAVnerGene:

AAV references are crucial for qPCR-based vector genome quantification and fundamental for ensuring accuracy in viral vector research and gene therapy development. Our AAV reference materials undergo extensive characterization, including full ratio (mass photometry), genome titer (ddPCR), capsid titer (ELISA), purity (SDS-PAGE silver staining), and tests for bioburden, endotoxin, and mycoplasma. Quantification is based on reference material from ATCC. These thoroughly analyzed particles provide precise genome titers, well-defined full-to-empty capsid ratios, and accurate capsid titers. This comprehensive characterization is essential for standardizing measurements, ensuring consistency across experiments, and delivering reliable data for gene therapy applications and other research involving AAV vectors.

AAVnerGene provide three types of AAV references: AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying  AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genomes.

 

AAV References Serotype Genome Production Plasmids
 AAV References (Full-2.8kb) AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eB Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb
 AAV References (Full-4.3kb) AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eB Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpA-4.3kb pAAVone-AAVs-CMV-EGFP-4.3kb
AAV References (Truly-Empty) AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eB No mini-pHelper-AAVs
AAV References (Byproduct-Empty) AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV2-Retro, AAV-DJ, and AAV-PHP.eB Partial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb

 

 

AAV references (Full) with different AAV serotypes

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Volume

100 µL, 1000 µL, 200 µL, 500 µL

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