AAVnerGene is dedicated to advancing the field of gene therapy through innovative AAV technologies and solutions. Based on our team’s experience and expertise in AAV gene therapy, AAVnerGene offers a range of services to support researchers and companies in developing and advancing their gene therapy projects. These services include AAV vector design, packaging and characterization, AAV library construction, production and screening, as well as barcoding technologies to enable efficient comparison and selection of promoters, enhancers, and AAV capsid variants for specific applications.
AAVnerGene offers AAV vector design and optimization services to help customers improve the specificity, potency, and safety of their AAV vectors. Our team of experts can help customers select appropriate AAV capsid, AAV plasmid backbone, promoters, enhancers, and regulatory elements to achieve optimal transgene expression in their target cells and tissues.
Highlights
- Diverse AAV vector backbones for different AAV production system, including pAAVone(for AAVone system), pAAVdual(for AAVdual system) and regular pAAV backbone(for triple plasmid system), for both regular single strand AAV(ssAAV) and self-complementary AAV(scAAV).
- Extensive collection of vector components, including ITRs, promoters, enhancers, transgenes, polyAs, and epitope tags.
- Comprehensive AAV vector design guides that cover AAV vector production systems, AAV capsids, and AAV vector components.
- To keep costs affordable, optimize your production processes and source materials in bulk where possible;
- Clone any custom fragments to any DNA vectors.
- Comprehensive downstream services, such as plasmid DNA preparation and AAV packaging.
- Short turnaround
Services | Description | Price | Turnaround |
Typical gene synthesis | Regular gene synthesis | $0.20/bp | 1-2 weeks |
Difficulty gene synthesis | GC-rich, high secondary structure | $0.35/bp | 2-3 weeks |
Plasmid Clone | Cloning any fragments into AAV backbone | $300 | 1-2 weeks |
Mini Prep | 10 ug plasmid endotoxin free plasmid | $100 | 3 days |
Midi Prep | 100 ug of endotoxin free plasmid | $150 | 3-5 days |
Maxi Prep | 400 ug of endotoxin free plasmid | $200 | 3-5 days |
Mega Prep | 2000 ug of endotoxin free plasmid | $500 | 3-5 days |
Giga Prep | 10000 ug of endotoxin free plasmid | $1,000 | 5-7 days |
AAV Packaging Services
AAVnerGene provides consistent, high quality, and affordable AAV vector package services to customers. AAVnerGene has created a novel mini-pHelper plasmid that has smaller in size and has fewer unnecessary sequences, which can reduce potential toxicity and improve transfection efficiency. The mini-pHelper has been optimized for high-yield AAV production, resulting in higher purity and quality of the AAV vectors produced. Moreover, AAVnerGene also developed high efficient AAV production systems, AAVone and AAVdual. Users can choose a system fit your needs.
We offer comprehensive solutions for AAV vectors, with:
- 1000+ AAV serotypes, variants available in-house
- Custom production runs available
- Both adherent and suspension cells, HEK293, HEK293T and AAVhigh-HEK293T production cells
- High production capacity, up to 1e16 gc scale
- CsCl, iodixanol gradient ultracentrifugation purification or AAV-X resin purification
- Over 85% ratio of full to empty capsids with high infectivity
- Friendly, responsive customer services
- Competitive pricing and fast turnaround
Scale | Titer | Volume | Price | Turnaround |
1e12 GC | 1e13 GC/ML | 1 X100 ul | $1,200 | 2 weeks |
5e12 GC | 1e13 GC/ML | 2 X 250 ul | $1,600 | 2 weeks |
1e13 GC | 1e13 GC/ML | 2 X 500 ul | $2,400 | 2 weeks |
2e13 GC | 1e13 GC/ML | 2 X 1000 ul | $4,200 | 2 weeks |
5e13 GC | 1e13 GC/ML | 5 X 1000 ul | $7,800 | 2-3 weeks |
1e14 GC | 1e13 GC/ML | 10 X 1000 ul | $11,800 | 2-3 weeks |
2e14 GC | 1e13 GC/ML | 20 X 1000 ul | $17,800 | 2-3 weeks |
5e14 GC | 1e13 GC/ML | 50 X 1000 ul | $42,800 | 3-4 weeks |
1e15 GC | 1e13 GC/ML | 100 X 1000 ul | $79,800 | 3-4 weeks |
>1e15 GC | TBD | TBD | TBD | TBD |
AAV Characterization Services
AAVnerGene offers standard quality control testing for AAV vectors, which includes vector genome titration, and purity analysis. In addition to these basic tests, the company also offers advanced testing services such as genome identity verification, toxicity testing, endotoxin or mycoplasma level testing, empty/full ratio determination, and more. These advanced tests can provide a more detailed understanding of the quality and safety of AAV vectors and help customers to select the best vectors for their specific applications.
QC | Method | Turnaround | Price | Sample Requirement |
Genome Titration | ddPCR/qPCR | 3-5 day | $200/sample | *Primer/probe sequences; ~20μl of samples |
Capsid Titration | ELISA | 3-5 day | $400/sample | *Serotypes and buffers; ~20μl of samples |
Purity Analysis | SDS-PAGE | 3-5 day | $200/sample | ~20μl of sample with titer >1E+12vg/ml |
Full/Empty Ratio | AUC | 3-5 day | $2000/sample | ~400μl of sample with titer >1E+12vg/ml |
Endotoxin | LAL assay | 3-5 day | $200/sample | ~20μl of samples |
Endotoxin Removal | Resin-PMB | 3-5 day | $200/sample | ~20μl of samples |
Bioburden Testing | Direct plating | 3-5 day | $200/sample | ~20μl of samples |
Mycoplasma Testing | PCR | 3-5 day | $200/sample | ~20μl of samples |
Vector Genome Identity | PacBio Seq | 4 weeks | Request | ~100μl of sample with titer >1E+12vg/ml |
AAV Library Construction Services
AAVnerGene offers two different AAV capsid libraries and its related services:
- AAV Capsid Library(I): is build on our ATHENA I platform. It is a known capsid library used to systematically compare and select the best AAV serotypes or variants. In the AAV capsid library(I), each capsid variant corresponds to three different DNA barcoded genomes with the same reporter. The unique barcode is located between the coding sequence and the Poly-A signal. During AAV production, one capsid and assigned 3 barcode plasmids were co-transfected to make one specific capsid AAV vector. All the AAV vectors were harvested, purified, and tittered, either pool or separately. By comparing the DNA or RNA levels of the reporter gene with NGS targeting the BCs, researchers can determine which AAV capsid variant is the most efficient for their specific application.
- AAV Capsid Library(II): is build on our ATHENA II platform. It is a random peptide insert library used to evolute novel AAV capsid with tissue specific tropism. In this library, random peptide sequences are inserted into variable sites of the AAV capsid protein. These peptides can potentially alter the properties of the capsid, such as its tropism, transduction efficiency, and immunogenicity, and thus affect its utility for gene therapy applications. By screening the library, researchers can identify novel capsids with improved properties and select the ones that are optimal for their specific therapeutic applications.
AAV Capsid Library(I) | AAV Capsid Library(II) | |
Library type | ATHENA IKnown AAV capsid collection library | ATHENA IIRandom peptide insert library |
Purpose | Evaluate different AAV capsids from known serotypes/variants | Create and evolve novel AAV capsids |
Complexity | Low(~1000 different AAV variants) | High(>1e9 variants) |
Reporter | CAG-EGFP | CAG/P40-Cap |
DNA barcodes | One capsid corresponding 3 different specific DNA barcodes | Random peptides themselves |
Production condition | Triple-plasmid transfection as usual | Low copy number of library plasmid plus normal pHelper and pRep plasmid |
Selection method | NGS (~100M) | NGS(usually>100M) |
Premade library | Common, Lung, BBB, etc. | AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh74 |
Library Construction services | Yes | Yes |
Selection services | Yes | Yes |
Prices | Based on request | Based on request |
AAV Library Production Services
Producing high-quality and diverse AAV libraries, especially the AAV Capsid Library(II), can be technically challenging. AAV library variants are prone to cross-packaging and capsid mosaic formation when produced at high plasmid levels. Producing an AAV capsid library in low copy number can help reduce the cross packaging and capsid mosaic formation. However, reduce the library plasmid copy number during the transfection would introduce another challenge, generating a large number of AAV vectors efficiently and reproducibly.
By using the mini-pHelper-Rep plasmid and high efficiency suspension HEK 293T cell lines, AAVnerGene was able to make AAV capsid library(II) production more accessible and affordable for researchers and biotech companies.
Scale | Packaging Conditions | Price | Turnaround |
2.00E+12 | 1000 copies/cell | $11,800 | 2-3 weeks |
200 copies/cell | $19,800 | 2-3 weeks | |
100 copies/cell | $29,800 | 2-3 weeks | |
<50 copies/cell | Please call to discuss | ||
2.00E+13 | 1000 copies/cell | $48,800 | 3-4 weeks |
200 copies/cell | $98,000 | 3-4 weeks | |
100 copies/cell | $148,800 | 3-4 weeks | |
<50 copies/cell | Please call to discuss | ||
2.00E+14 | Please call to discuss | Please call to discuss | |
AAV Capsid Selection and Evolution Services
AAVnerGene offers a comprehensive range of services related to AAV capsid assessment and evolution, catering to both in vitro and in vivo research needs.
- AAVnerGene offers capsid evaluation services for AAV Capsid Library(I) and capsid evolution services for AAV Capsid Library(II).
- AAVnerGene offers different models for AAV evaluation and evolution, from in vitro (primary cells, organoids) to in vivo (mouse, rat, and NHP).
- Please contact us to get a quote, customer@aavnergene.com.
