Name: pAAVdual-sc-CMV-EGFP - AAVnerGene
SKU: SL023001
Availability: InStock

pAAVdual-sc-CMV-EGFP

pAAVdual-sc-CMV-EGFP is used to produce scAAV-CMV-EGFP virus using AAVnerGene’s novel AAVdual production system. In this plasmid, the self-complementary AAV genome with a CMV promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. scAAV-CMV-EGFP virus can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.

The CMV promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.

SKU: SL023001
AAV Cassette: scAAV-CMV-EGFP
AAV Genome Size: 2165 bp
Plasmid Size: 10678 bp
Plasmid Backbone: pAAVdual-sc
AAV Genome Type: scAAV
ITRs: mITR (72 bp) + R-ITR (130 bp)
Promoter: CMV
Transgene: EGFP
Bacterial Resistance(s) : Kana
Storage: -20°C for long term (> 1 year)
Shipping: Dry ice.
Notice to Purchaser: MTA is required. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids, are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.

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pAAVdual-sc plasmids are used to produce self-complementary AAV (scAAV) viruses using AAVnerGene’s novel AAVdual System. In this series plasmids, the scAAV genomes are cloned into our novel Ad helper plasmid, mini-pHelper. scAAV viruses can be generated by co-transfection of pAAVdual-sc plasmids with the regular AAV helper plasmids.

pAAVdual and pAAVdual-sc plasmids with other promoters are available at AAVnerGene:

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Advantages of AAVnerGene’s AAVdual system compared with traditional triple plasmid system:

1.Increase AAV yield up to 250%.
2.Reduce plasmid number from 3 to 2.
3.Reduce batch variations.
4.Simply the plasmid ratio optimization processes.
5.Compact plasmid size(8.5kb plasmid backbone + AAV genome) and comparable plasmid yields.
6.Comparable or increased full-to-empty ratio.
7.Similar viral activity, capsid and genomic components.
8.Similar levels of rcAAV, hcDNAs and producer plasmid contaminations.
9.The same upstream and downstream processes, analytical release or characterization assays.