AAV9-TRE-DA2h viruses are ready to use AAV vectors carrying TRE-DA2h expression cassette. AAVnerGene also offers TRE-DA2h vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The TRE promoter (Tetracycline-Responsive Element) is a synthetic DNA sequence used in genetic engineering to control gene expression, featuring multiple copies of the tetO (tetracycline operator) site fused to a minimal promoter, allowing precise turning on/off of downstream genes with tetracycline or its derivatives like doxycycline, forming the widely used Tet-Off and Tet-On systems for inducible gene.
DA2h is a second‑generation, high‑affinity dopamine GRAB sensor engineered from the human D2 dopamine receptor with a cpEGFP inserted in the third intracellular loop. It was optimized to detect lower dopamine concentrations with high sensitivity, providing nanomolar‑range affinity and robust EGFP fluorescence responses to extracellular dopamine.
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.
Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
SDS-PAGEGel: Assess the purity of the AAV viruses is great than 95%.
Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
$399.00–$2,799.00Price range: $399.00 through $2,799.00Select options
This product has multiple variants. The options may be chosen on the product page
$399.00–$2,799.00Price range: $399.00 through $2,799.00Select options
This product has multiple variants. The options may be chosen on the product page
$498.00–$3,598.00Price range: $498.00 through $3,598.00Select options
This product has multiple variants. The options may be chosen on the product page
$399.00–$2,799.00Price range: $399.00 through $2,799.00Select options
This product has multiple variants. The options may be chosen on the product page
