AAV-Myog-EGFP
Price range: $299.00 through $1,799.00
AAV-Myog-EGFP series viruses are ready to use AAV vectors carrying Myog-EGFP expression cassette. AAVnerGene offers AAV-Myog-EGFP vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The Myog promoter refers to the promoter region of the Myogenin (Myog) gene, which plays a critical role in muscle development, particularly in the differentiation of skeletal muscle cells (myogenesis). Myogenin is a member of the myogenic regulatory factors (MRFs), a group of transcription factors essential for muscle cell differentiation. The Myog promoter is a muscle-specific regulatory element that drives gene expression during the differentiation of skeletal muscle cells. It is a vital tool in muscle development research, gene therapy for muscle diseases, and the creation of transgenic models to study myogenesis and muscle function. Its specificity to muscle tissue, particularly during development, makes it invaluable for targeting gene expression in studies focused on skeletal muscle formation and regenerative medicine.
By employing EGFP as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.
- AAV Plasmids: pAAVdual-Myog-EGFP + pRCap-AAVs
- AAV Genome: AAV-Myog-SV40intron-EGFP-WPRE3-SV40polyA
- AAV Genomic Type: Single strand AAV (ssAAV)
- AAV Genomic Size: 2771 bp
- AAV Gene Specific Titer: 1X10^13 VG/ml
- AAV Full Percentage: >85%
- AAV Production System: AAVdual system
- AAV Production Cells: HEK 293T
- AAV Purification: Two rounds of CsCl ultracentrifuge
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
- Shipping: Dry ice
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| AAV Serotype | AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74 |
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