AAV-hBEST1-EGFP
Price range: $299.00 through $1,799.00
AAV-hBEST1-EGFP series viruses are ready to use AAV vectors carrying hBEST1-EGFP expression cassette. AAVnerGene offers AAV-hBEST1-EGFP vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The hBEST1 promoter refers to the promoter region of the human Bestrophin 1 (BEST1) gene, which is primarily expressed in the retinal pigment epithelium (RPE) of the eye. The BEST1 gene encodes a protein that functions as a chloride channel and plays a crucial role in maintaining the health and function of the RPE, which is essential for normal vision. The hBEST1 promoter is a retinal pigment epithelium-specific regulatory element that drives gene expression in the RPE, a vital layer of the retina. It is a crucial tool in retinal gene therapy, ophthalmology research, and the development of transgenic models for studying the RPE and related retinal diseases. Its specificity to the RPE makes it invaluable for targeting gene expression in studies focused on retinal health, disease, and therapy.
By employing EGFP as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.
- AAV Plasmids: pAAVdual-hBEST1-EGFP + pRCap-AAVs
- AAV Genome: AAV-hBEST1-SV40intron-EGFP-WPRE3-SV40polyA
- AAV Genomic Type: Single strand AAV (ssAAV)
- AAV Genomic Size: 2255 bp
- AAV Gene Specific Titer: 1X10^13 VG/ml
- AAV Full Percentage: >85%
- AAV Production System: AAVdual system
- AAV Production Cells: HEK 293T
- AAV Purification: Two rounds of CsCl ultracentrifuge
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
- Shipping: Dry ice
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| AAV Serotype | AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74 |
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