AAV Plasmids
AAVnerGene offers a comprehensive range of AAV plasmids (pAAVone, pAAVdual, and pAAVtri) tailored for various AAV production systems, including AAVone®, AAVdual®, and AAVtri. These flexible systems cater to diverse vector designs & production requirements, optimizing efficiency and scalability for gene therapy. We also offer a diverse range of AAV barcode plasmids, featuring various backbones, promoters, reporters, and barcodes to meet your specific research needs, to make your own AAV Capsid Barcode Kits.
AAV Plasmids for AAV Production
To address AAV production challenges, AAVnerGene has developed innovative technologies aimed at improving traditional triple-plasmid AAV production systems packaging efficiency:
- AAVtri (Triple-Plasmid System)
- mini-pHelper-1.0 – A compact adenoviral helper plasmid (8.4 kb) delivering E2A, E4orf6, and VA RNA for improved packaging efficiency over traditional Ad helper plasmids.
- pRCap – An optimized AAV helper plasmid featuring a modified Rep expression cassette, enhancing packaging efficiency compared to standard AAV2 Rep.
- pAAVtri – Regular AAV cis plasmid carrying two ITRs and GOI expression cassette.
Building on the mini-pHelper-1.0 and pRcap, AAVnerGene has introduced two advanced systems:
- AAVone® (Single-Plasmid System)
- Combines all essential components—Ad helper genes (E2A, E4orf6, VA RNA), AAV helper genes (Rep/Cap), and the AAV vector genome—into a single plasmid (pAAVone) [Yang et al., 2025].
- Achieves high-yield AAV production (AAV9 >5 × 10¹⁵ vg/L) in HEK293 cells.
- AAVdual® (Two-Plasmid System)
- Integrates the AAV genome and transgene into the optimized mini-pHelper-1.0 backbone (pAAVdual) [Yang et al., 2025].
- Balances high AAV productivity with AAV capsid flexibility.
These systems significantly streamline AAV production, reducing complexity and improving scalability for both research and clinical applications.
| Production System | Plasmid Backbone | Examples | Ad Genes | Rep/Cap | Genome |
| AAVone® System | pAAVone | pAAVone-AAV9-CMV-EGFP | Yes | Yes | ssAAV |
| pAAVone-sc | pAAVone-sc-AAV9-CMV-EGFP | Yes | Yes | scAAV | |
| AAVdual® System | pAAVdual | pAAVdual-CMV-EGFP | Yes | No | ssAAV |
| pAAVdual-sc | pAAVdual-sc-CMV-EGFP | Yes | No | scAAV | |
| AAV helper | pRCap-AAV9 | No | Yes | scAAV | |
| AAVtri System | pAAVtri | pAAVtri-CMV-EGFP | No | No | ssAAV |
| pAAVtri-sc | pAAVtri-sc-CMV-EGFP | No | No | scAAV | |
| Ad helper | mini-pHelper-1.0 | Yes | No | No | |
| AAV helper | pRCap-AAV2 | No | Yes | No |
pAAVone Plasmids with Different AAV Serotypes
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pAAVdual Plasmids with Different Promoters
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pAAVtri, mini-pHelper-1.0 and pRCap Plasmids
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AAV Barcode Plasmids
AAV barcode reporter plasmids are essential for generating AAV Capsid Barcode Kits. Each plasmid contains a unique barcode strategically positioned between the WPRE enhancer and the PolyA sequence. At AAVnerGene, we offer a diverse range of AAV barcode plasmids featuring various backbones, promoters, reporters, and barcodes to meet your specific research needs.
| Backbone | Product Name | SKU | Barcode Design | Request Plasmids |
| pAAVtri | pAAV-CAG-EGFP-BC-N25 | SA005001A | N25 (NNNNNNNNNNNNNNNNNNNNNNNNN) | pRCap+ mini-pHelper |
| pAAV-CAG-EGFP-BC-2XN12 | SA005001B | 2XN12(NNNNNNNNNNNNCGGAAATACGATGTCGGGANNNNNNNNNNNN | pRCap + mini-pHelper | |
| pAAVdual | pAAVdual-CMV-mCherry-BC-N25 | SA001006A | N25 (NNNNNNNNNNNNNNNNNNNNNNNNN) | pRcap |
| pAAVdual-sc-CMV-mCherry-BC-N25 | SA023006A | N25 (NNNNNNNNNNNNNNNNNNNNNNNNN) | pRcap |