AAV-sgRNA Selection Kits

The AAV-sgRNA selection kits include 4 sgRNAs, 1 control sgRNA, and a GOI-mCherry fusion reporter plasmid, providing a comprehensive toolkit for effective gene knockdown and real-time monitoring of transduction and expression. The sgRNAs are expressed by the U6 promoter, while the Cas proteins are controlled by a small but strong ubiquitous promoter, all within a single plasmid. Additionally, the GOI-mCherry fusion reporter plasmid is included to monitor gene knockdown efficiency. Customers only need to provide the GOI information or sequence to be silenced and select the gene editing systems. Co-transfection of the AAV-sgRNA plasmids and the GOI-mCherry reporter plasmid allows researchers to quickly assess the effectiveness of each sgRNA.