pAAVtri-sc-CMV-EGFP

pAAVtri-sc-CMV-EGFP  is used to produce scAAV-CMV-EGFP virus using AAVnerGene’s novel AAVtri System or traditional tri-plasmid systems. In this plasmid,  the  regular self-complementary  AAV genome  with a CMV promoter and an EGFP reporter is cloned into pAAVtri-sc backbone.  scAAV-CMV-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper  (pRCap) plasmids, carrying AAV2 rep gene and different cap genes and mini-pHelper or other Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.

The CMV promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.

• AAV Cassette: scAAV-CMV-EGFP
• AAV Genome Size:  4,237 bp for scAAV genome
• Plasmid Sequence: pAAVtri-sc-CMV-EGFP
• Plasmid Size: 4,609 bp
• Plasmid Backbone: pAAVtri-sc
• AAV Genome Type: scAAV
• ITRs:  L-ITR (106 bp) + R-ITR (130 bp)
• Promoter: CMV
• Transgene: EGFP
• Bacterial Resistance(s) : Kana
• Storage:  -20°C for long term (> 1 year)
• Shipping: Dry ice.
• Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
• AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
• AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.