pAAVone-sc-CMV-EGFP Series are plasmids used to produce sc-AAV-CMV-EGFP with different AAV serotypes using AAVnerGene’s novel AAVone production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and a self-complementary AAV-CMV-EGFP genome. To produce sc-AAV-CMV-EGFP with a certain serotype, users need select the pAAVone-sc plasmid carrying the corresponding capsid.
Premade serotypes include AAV1, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, AAV13, AAVDJ, AAVrh.10, and AAVrh74. For any other AAV serotypes or variants, please contact us and we will produce them within 2 weeks.
pAAVone-sc-CMV-EGFP Series Plasmids:
| Image | Name | Price | Buy |
|---|---|---|---|
 | pAAVone-sc-AAV-PHP.eB-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV1-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV10-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV11-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV12-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV13-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV2-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV3B-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV4-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV5-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV6-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV7-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV8-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAV9-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAVrh.10-CMV-EGFP | $999.00 – $1,899.00 | |
| pAAVone-sc-AAVrh74-CMV-EGFP | $999.00 – $1,899.00 |
pAAVone-CMV-EGFP Series Plasmids:
| Image | Name | Price | Buy |
|---|---|---|---|
 | pAAVone-CMV-EGFP Series | $999.00 – $1,899.00 |
pAAVone-CMV-mCherry Series Plasmids:
| Image | Name | Price | Buy |
|---|---|---|---|
 | pAAVone-CMV-mCherry Series | $999.00 – $1,899.00 |
Advantages of AAVone system compared with traditional triple plasmid system:
1. Increase AAV yield up to 400%.
2. Reduce plasmid number from 3 to 1.
3.Reduce plasmid amount from 1 to 0.25 ug/1e6 cells.
4.Reduce batch variations.
5.Without need the plasmid ratio optimization steps.
6.Compact plasmid size(16±2kb) and comparable yields.
7.Comparable or increased full-to-empty ratio.
8.Similar viral activity, capsid and genomic components.
9.Similar levels of rcAAV, hcDNAs and producer plasmid contaminations.
10.The same upstream and downstream processes, analytical release or characterization assays.
In summary, AAVone system offers a simple, low cost, but high consistency and high efficiency for production of AAV vectors, especially for large scale and GMP grade AAV production.
Workflow of AAVone production system:
AAVone system increase AAV yield up to 400% compared with traditional triple plasmid system:
AAVone system generated high quality AAV vectors:
