pAAVdual-mTyr-EGFP

pAAVdual-mTyr-EGFP  is used to produce AAV-mTyr-EGFP virus using AAVnerGene’s novel AAVdual production system. In this plasmid,  the  regular single strand AAV genome  with a mTyr promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper.  AAV-mTyr-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper  (pRCap) plasmids, carrying AAV2 rep gene and different cap genes,  without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.

The mTyr promoter refers to the promoter region of the mouse Tyrosinase (Tyr) gene, which is crucial for driving gene expression specifically in melanocytes, the cells responsible for producing melanin in the skin, hair, and eyes. Tyrosinase is a key enzyme in the melanin biosynthesis pathway, and the mTyr promoter is often used in research to study pigmentation, melanoma, and other melanocyte-related processes.The mTyr promoter is a melanocyte-specific regulatory element that drives gene expression in pigment-producing cells. It is an essential tool in pigmentation research, melanoma studies, and the development of transgenic models for exploring melanocyte function and related diseases. Its specificity to melanocytes makes it invaluable for targeting gene expression in studies focused on melanin production, pigmentation, and skin-related disorders.

• AAV Cassette: AAV-mTyr-EGFP
• AAV Genome Size: 2167 bp
• Plasmid Size: 10641 bp
• Plasmid Backbone: pAAVdual
• AAV Genome Type: ssAAV
• ITRs:  L-ITR (119 bp) + R-ITR (130 bp)
• Promoter: mTyr
• Transgene: EGFP
• Bacterial Resistance(s) : Kana
• Storage:  -20°C for long term (> 1 year)
• Shipping: Dry ice.
• Notice to Purchaser:  MTA is required. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
• Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
• AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
• AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.