pAAVdual-EF1a-Gluc

pAAVdual-EF1a-Gluc  is used to produce AAV-EF1a-Gluc virus using AAVnerGene’s novel AAVdual production system. In this plasmid,  the  regular single strand AAV genome  with a EF1a promoter and an Gluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper.  AAV-EF1a-Gluc viruses can be generated by co-transfection of this plasmid with regular AAV helper  (pRCap) plasmids, carrying AAV2 rep gene and different cap genes,  without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.

The EF1a promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.

Gaussia luciferase (Gluc) is a highly sensitive bioluminescent reporter protein commonly used in biological research to monitor gene expression, track cellular processes, or assess promoter activity in real time. By employing Gluc as a reporter, researchers gain the ability to effortlessly quantify the transduction efficiency of AAV vectors within target cells. The AAV-Gluc system allows researchers to perform in vivo and in vitro bioluminescent assays.

• AAV Cassette: AAV-EF1a-Gluc
• AAV Genome Size: 2649 bp
• Plasmid Size: 11082 bp
• Plasmid Backbone: pAAVdual
• AAV Genome Type: ssAAV
• ITRs:  L-ITR (119 bp) + R-ITR (130 bp)
• Promoter: EF1a
• Transgene: Gluc
• Bacterial Resistance(s) : Kana
• Storage:  -20°C for long term (> 1 year)
• Shipping: Dry ice.
• Notice to Purchaser:  MTA is required. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
• Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
• AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
• AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.