pAAVdual-CMV-mCherry-BC-N25
Price range: $100.00 through $1,000.00
pAAVdual-CMV-mCherry-BC-N25 plasmids are used to generate AAV Capsid Barcode Kits and AAV Capsid Barcode Libraries. This plasmid has AAV-CMV-SV40intron-mCherry-WPRE-BC-N25-bGHpolyA expression cassette. The CMV promoter is known for strong and ubiquitous expression in various cell types. The presence of the mCherry reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes. WPRE3 enhancer and SV40PolyA can enhance mCherry expression and stabilize mRNA. The random N25 BCs are located between WPRE3 and SV40PolyA. .
Each plasmid in this series contains a unique DNA barcode (BC), allowing for the identification and tracking of individual AAV capsid variants in the kits or libraries. The random N25 BCs are located between WPRE and bGHPolyA.
- Plasmid Name: pAAVdual-CMV-mCherry-BC-N25
- Plasmid Size: 11257 bp
- Plasmid Backbone: pAAVdual
- AAV Cassette:AAV-CMV-SV40intron-mCherry-WPRE-BC-N25-bGHpolyA, 2850 bp
- Final AAV Genome Size: 2891 bp
- BC design: N25
- AAV Genome Type: ssAAV
- ITRs: L-ITR (119 bp) + R-ITR (130 bp)
- Promoter: CMV
- Transgene: mCherry
- Bacterial Resistance(s): Amp
- Storage: -20°C for long term (> 1 year)
- Shipping: Dry ice.
- Notice to Purchaser: MTA is required, and a fee of $1,000 will be applied for the use of the pAAVdual backbone if this is your first time utilizing our AAVdual system. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids, are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
Related Services:
- Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
- AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
- AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.
- Custom AAV Capsid Barcode Kits: Based on our ATHENA-I platform, we can help to make thier own AAV capsid barcode kits or libraries. Customers can choose their own AAV capsids, promoters, reporters, and other elements.
| Amount | 10 μg, 100 μg, 1 mg, 10 mg |
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