pAAV-CAG-EGFP-BC-2XN12
Price range: $100.00 through $1,000.00
pAAV-CAG-EGFP-BC-2xN12 plasmids are used to generate AAV Capsid Barcode Kits and AAV Capsid BC-Libraries. This plasmid has AAV-CAG-EGFP-WPRE-BC-2xN12-bGHpolyA expression cassette. The CAG promoter is known for strong and ubiquitous expression in various cell types. The presence of the EGFP reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes. WPRE enhancer and bGH polyA can enhance EGFP expression and stabilize mRNA.
Each plasmid in this series contains a unique DNA barcode (BC), allowing for the identification and tracking of individual AAV capsid variants in the kits or libraries. The random 2xN12 BCs are located between WPRE and bGHPolyA.
- Plasmid Name: pAAV-CAG-EGFP-BC-2xN12
- Plasmid Size: 6150 bp
- Plasmid Backbone: pAAV
- AAV Cassette: AAV-CAG-EGFP-WPRE-BC-2xN12-bGHpolyA, 3019 bp
- Final AAV Genome Size: 3049 bp
- BC design: NNNNNNNNNNNNACGGAAATACGATGTCGGGANNNNNNNNNNNN
- AAV Genome Type: ssAAV
- ITRs: L-ITR (130 bp) + R-ITR (130 bp)
- Promoter: CAG
- Transgene: EGFP
- Bacterial Resistance(s): Amp
- Storage: -20°C for long term (> 1 year)
- Shipping: Dry ice.
Related Services:
- Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
- AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
- AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.
- Custom AAV Capsid Barcode Kits: Based on our ATHENA-I platform, we can help to make thier own AAV capsid barcode kits or libraries. Customers can choose their own AAV capsids, promoters, reporters, and other elements.
| Amount | 10 μg, 100 μg, 1 mg, 10 mg |
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