AAV9 Reference-2.8kb
Price range: $399.00 through $2,799.00
The AAV9 reference-2.8kb material contains a 2.8kb AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA genome in AAV9 capsid, with a genome titer (EGFP targeting) of 1X10^13 VG/ml. This reference is produced using the AAVone system with the pAAVone-AAV9-CMV-EGFP-2.8kb plasmid. AAV9 is one of the most widely used adeno-associated virus serotypes, especially known for its ability to efficiently transduce a broad range of tissues, including the central nervous system (CNS), liver, heart, and muscle. AAV9 has garnered considerable attention in the field of gene therapy due to its unique ability to cross the blood-brain barrier (BBB) and efficiently transduce neurons following systemic administration. This distinctive feature makes it particularly well-suited for both peripheral and central nervous system (CNS) gene therapy applications. A prominent example of its use is in Zolgensma (Onasemnogene Abeparvovec), a groundbreaking treatment for spinal muscular atrophy (SMA). SMA is a severe genetic disorder characterized by the loss of motor neurons and progressive muscle weakness.
- AAV Serotype: AAV9
- AAV Genome: AAV-CMV-SV40I-EGFP-WPRE-hGHpA-2.8k
- AAV Genome Type and Size: ssAAV, 2843 bp,
- Promoter: CMV
- Intron: SV40 Intron
- Reporter: EGFP
- Enhancer: WPRE
- PolyA: hGHpolyA
- AAV Genome Titer: 1X10^13 VG/ml, targeting EGFP
- Full particle ratio: >85% by mass photometry
- Producer Plasmid: pAAVone-AAV9-CMV-EGFP-2.8kb
- Producer Cell line: Suspension HEK 293T
- Purification: AAVx followed by one round of CsCl ultracentrifuge.
- Endotoxin: < 1.0 EU/ml
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
- Storage: -80°C for long term (> 1 year).
- Shipping: Dry ice.
AAV References at AAVnerGene:
AAV references are crucial for qPCR-based vector genome quantification and fundamental for ensuring accuracy in viral vector research and gene therapy development. Our AAV reference materials undergo extensive characterization, including full ratio (mass photometry), genome titer (ddPCR), capsid titer (ELISA), purity (SDS-PAGE silver staining), and tests for bioburden, endotoxin, and mycoplasma. Quantification is based on reference material from ATCC. These thoroughly analyzed particles provide precise genome titers, well-defined full-to-empty capsid ratios, and accurate capsid titers. This comprehensive characterization is essential for standardizing measurements, ensuring consistency across experiments, and delivering reliable data for gene therapy applications and other research involving AAV vectors.
AAVnerGene provide three types of AAV references: AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genomes.
| AAV References | Serotype | Genome | Production Plasmids |
| AAV References-2.8kb | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb | pAAVone-AAVs-CMV-EGFP-2.8kb |
| AAV References-4.3kb | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpA-4.3kb | pAAVone-AAVs-CMV-EGFP-4.3kb |
| AAV References (Truly-Empty) | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | No | mini-pHelper-AAVs |
| AAV References (Byproduct-Empty) | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | Partial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb | pAAVone-AAVs-CMV-EGFP-2.8kb |
AAV references (Full) with different AAV serotypes
| Name | SKU | Price | Buy |
|---|
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
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