AAV9-GfaABC1D-ATP1.0mut viruses are ready to use AAV vectors carrying GfaABC1D-ATP1.0mut expression cassette. AAVnerGene also offers GfaABC1D-ATP1.0mut vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The GfaABC1D promoter is a specific regulatory DNA sequence that controls the expression of the GfaABC1D gene. This promoter is typically associated with driving gene expression in glial fibrillary acidic protein (GFAP)-expressing cells, particularly astrocytes in the central nervous system. The GfaABC1D promoter is a shortened version of the longer GFAP promoter, optimized for more specific or efficient expression in certain experimental contexts. This promoter is widely used in gene therapy, neuroscience, and molecular biology to target gene expression specifically to astrocytes. Researchers often use it in experiments where controlling gene expression in these glial cells is crucial, such as in studies of neurodegenerative diseases, brain injury, or astrocyte function.
ATP1.0mut is an engineered, ATP‑insensitive mutant version of the GRAB ATP1.0 sensor, designed to serve as a negative control in experiments measuring extracellular ATP. It retains the structural framework of the P2Y1‑based cpGFP sensor but contains mutations that prevent ATP‑induced conformational changes, resulting in no fluorescence response upon ATP binding.
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.
Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
SDS-PAGEGel: Assess the purity of the AAV viruses is great than 95%.
Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
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