AAV9-CAG-5-HT1.0 (5-HT2.1)
Price range: $199.00 through $249.00
AAV9-CAG-5-HT1.0 viruses are ready to use AAV vectors carrying CAG-5-HT1.0 expression cassette. AAVnerGene also offers AAV-CAG-5-HT1.0 vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The CAG promoter is a synthetic, hybrid and ubiquitous promoter widely used in molecular biology to drive high levels of gene expression in mammalian cells. It is particularly valued for its strength and broad activity across various cell types, making it a popular choice for applications such as gene therapy, transgenic animal models, and research studies that require robust and consistent gene expression. It is composed of three key elements:
- CMV Immediate-Early Enhancer: Derived from the cytomegalovirus, this enhancer sequence provides strong and ubiquitous transcriptional activation in a variety of cell types.
- Chick Beta-Actin Promoter: The core promoter region is derived from the chicken beta-actin gene, which is known for its high expression levels in many cell types, particularly in the context of gene expression in vertebrates.
- Rabbit Beta-Globin Intron: An intronic sequence from the rabbit beta-globin gene is included to enhance transcriptional efficiency and mRNA stability, further boosting expression levels.
The GRAB 5‑HT1.0 sensor is an engineered GPCR-based EGFP fluorescent probe that lights up when it detects serotonin. It’s fast, sensitive, and can be genetically targeted to specific neurons, enabling researchers to watch serotonin dynamics in the living brain with high precision.
- AAV Plasmids: pAAV-CAG-5-HT1.0
- AAV Genomic Type: Single strand AAV (ssAAV)
- AAV Gene Specific Titer: 1X10^13 VG/ml
- AAV Full Percentage: >85%
- AAV Production System: AAVtri system
- AAV Production Cells: HEK 293T
- AAV Purification: Iodixanol Gradient Ultracentrifugation
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
- Shipping: Dry ice
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.
Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 10 µL, 50 µL |
|---|---|
| AAV Serotype | AAV9 |
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