AAV-CaMKIIα0.36-Fluc-Virus-Map

AAV-CaMKIIα(0.36)-Fluc

Price range: $299.00 through $1,799.00

AAV-CaMKIIα(0.36)-Fluc series viruses are ready to use AAV vectors carrying CaMKIIα(0.36)-Fluc expression cassette. AAVnerGene offers AAV-CaMKIIα(0.36)-Fluc vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.

The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.

Firefly luciferase (Fluc) catalyzes the oxidation of its substrate, luciferin, in the presence of ATP and oxygen, producing light in the yellow-green spectrum. This bioluminescent reaction is widely used in biological research for real-time imaging, monitoring gene expression, and tracking various cellular processes. AAV-Fluc is a powerful tool for researchers who need to perform sensitive, non-invasive imaging and monitoring of gene expression and other cellular processes. Its high sensitivity, broad dynamic range, and compatibility with various research models make it an essential component of many bioluminescence-based assays and in vivo studies.

  • AAV Plasmids: pAAVdual-CaMKIIα(0.36)-Fluc + pRCaps
  • AAV Genome: AAV-CaMKIIα(0.36)-SV40intron-Fluc-WPRE3-SV40polyA
  • AAV Genomic Type: Single strand AAV (ssAAV)
  • AAV Genomic Size: 2928 bp
  • AAV Gene Specific Titer: 1X10^13 VG/ml
  • AAV Full Percentage: >85%
  • AAV Production System: AAVdual system
  • AAV Production Cells: HEK 293T
  • AAV Purification: Two rounds of CsCl ultracentrifuge
  • Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
  • Storage: -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks
  • Shipping: Dry ice
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SKU: DP008003 Categories: , , , , ,

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation.  The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:

  • Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
  • Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
  • Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
  • SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
  • Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.

 

Volume

100 µL, 1000 µL, 200 µL, 500 µL
AAV Serotype

AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74

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