Name: AAV ACTOne Gi-GPCR Assay Kit-CB2 - AAVnerGene
SKU: KI001-CB2
Availability: InStock

AAV ACTOne Gi-GPCR Assay Kit-CB2

The AAV ACTOne GPCR Assay Kit-CB2 is designed specifically for screening drugs/compounds that target the Cannabinoid Receptor 2 (CB2) by combination AAV and ACTOne technologies.

Cannabinoid Receptor 2 (CB2) is a type of G protein-coupled receptor (GPCR) primarily involved in the immune system and peripheral tissues. Unlike Cannabinoid Receptor 1 (CB1), which is predominantly found in the central nervous system, CB2 receptors are mainly expressed in immune cells, the spleen, tonsils, and other peripheral organs. They are also present in smaller amounts in the brain, particularly under conditions of inflammation or injury. CB2 is primarily coupled with the Gi/Go class of G proteins, which inhibit adenylate cyclase, leading to a decrease in cyclic AMP (cAMP) levels. Activation of CB2 receptors plays a significant role in modulating immune responses, reducing inflammation, and protecting tissues from damage during inflammatory processes. CB2 is a promising therapeutic target for conditions such as autoimmune diseases, chronic inflammatory disorders, and neurodegenerative diseases. Unlike CB1, CB2 activation does not produce psychoactive effects, making it an attractive focus for drug development.

This kit uses AAV vectors with a CMV promoter to co-express the CB2 and cyclic nucleotide-gated (CNG) channel, allowing researchers to conduct high-throughput screening and functional analysis of potential CB2-targeting compounds. The kit provides a sensitive and reliable method for evaluating the pharmacological properties of CB2 drugs, such as agonists and antagonists, in a live-cell environment.

SKU: KI001-CB2
Targeting GPCR: Cannabinoid Receptor 2 (CB2)，NP_001832
GPCR Type: Gi-GPCR
Kit components: AAV-CNG/AAV-CB2 and ACTOne MP Dye
Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks.
Shipping: Dry ice.

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The AAV ACTOne GPCR Assay Kits are live-cell-based cAMP biosensor systems designed for rapid and high-throughput functional screening of G-protein coupled receptors (GPCRs) by combination AAV. Kits are available for both Gi-GPCR and Gs-GPCR assays.

AAV ACTOne Gi-GPCR Assay Kits:

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AAV ACTOne Gs-GPCR Assay Kits:

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Advantages of AAV ACTOne GPCR Assay Kits:

Combined ACTOne and AAV Technologies
Transient Expression of CNG and GPCR
Real Time Assay on Living Cell
High Sensitivity and Big assay window
Both Kinetic and Endpoint Assay
Easy to use by mixing the AAVs with cells
Faster setup and time efficiency
Reduced risk of genetic drift,
Simplified workflows,
flexibility in dynamic control of expression
Low Cost: $498 for one plate

ACTOne GPCR Assay

ACTOne™ is the only high-throughput GPCR screening technology that can directly measure the intracellular changes of the secondary messenger cyclic AMP (cAMP) in living cells, in real-time. It uses a proprietary modified cyclic nucleotide-gated (CNG) channel, which is co-localized with adenylate cyclase at the plasma membrane, as a biosensor of cAMP activity. The CNG channel opens when the cAMP level near the plasma membrane increases, resulting in ion flux and cell membrane depolarization. The influx of cations through the CNG channel can be quantified using fluorescent ion indicators or membrane potential (MP) dyes. It provides information on real time intracellular cAMP changes and is highly sensitive. By combining kinetic and endpoint readouts, we are able to capture and analyze transient responses from endogenous GPCRs and weak responses caused by weak Gs or Gi coupled GPCR activities. Using ACTOne, we are able to detect the subcellular cAMP concentration changes directly caused by GPCR activation. Real-time kinetic readouts minimize artifacts, and provide greater content and more statistically relevant data. The intensity of signal increase caused by GPCR activation is directly related to the receptor number on cell surface. Using ACTOne assay, we were able to detect activities of some endogenous Gs coupled receptors in HEK293 cells that have not been reported in literature. In addition, we have also detected weak Gs coupled activity of a GPCR that was widely considered to be only linked to Gq coupled pathway. The ACTOne assay also provides a useful tool for GPCR de-orphanization.