AAV capsid libraries have been instrumental in the development of more efficient and targeted gene therapies. They allow researchers to explore the vast diversity of AAV capsids, leading to the discovery of novel variants that can overcome limitations and enhance the success of gene therapy approaches. These libraries are also valuable tools for understanding the biology of AAVs and how they interact with host cells.
AAVnerGene offers two types of premade AAV capsid libraries:
- AAV Capsid Library(I) is a known capsid library designed to compare and select the best AAV serotypes or variants.
- AAV Capsid Library(II) is a random peptide insert library used to evolute novel AAV capsid with tissue-specific tropism or other unique purposes.
AAV Capsid Library(I)
AAV capsid libraries(I) are known capsid library build on our ATHENA I platform. They are used to systematically evaluate different AAV serotypes or variants by using Barcode-Seq technology. In AAV Capsid Library(I), each capsid variant was assigned to three different DNA barcodes. By comparing DNA or RNA levels of the reporter gene with NGS targeting the DNA barcodes, researchers can determine which AAV capsid variant is the most efficient for their specific application.
AAVnerGene provides different tissue-specific AAV capsid libraries(I), which contains 16 common AAV serotypes in AAV capsid libraries(I)-Common and 9 selected tissue-targeting capsids. The standard kits are use CAG-EGFP as reporter cassettes. We also provide custom kit services, users can provide their own reporter systems and add any tissues specific capsids into the kits.
Common AAV Capsids: AAV1, AAV2, AAV3B, AAV4-Y729F, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh74, AAV-DJ, AAV2-Retro
|AAV Capsid Library(I)-BBB||AAV-B1, AAV-F, AAV-MaCPNS1, AAV-PHP.B, AAV-PHP.C2, AAV-PHP.eB, AAV-PHP.S, AAV.Cap-B22, AAV/Olig001.||$9,999.00 – $54,999.00||aav-b1 aav-f aav-macpns1 aav-php-b aav-php-c2 aav-php-eb aav-php-s aav-cap-b22 aav-olig001|
|AAV Capsid Library(I)-Common||No additional tissue-targeting capsids||$5,999.00 – $32,999.00||no-additional-tissue-targeting-capsids|
|AAV Capsid Library(I)-Lung||AAV2-ESGHGYF, AAV2-PRSTSDP, AAV2-Y444F-Y500F, AAV2.5T, AAV2H22, AAV5-PK2, AAV6.2, AAV6.2FF, AAV9.452sub.LUNG1||$9,999.00 – $54,999.00||aav2-esghgyf aav2-prstsdp aav2-y444f-y500f aav2-5t aav2h22 aav5-pk2 aav6-2 aav6-2ff aav9-452sub-lung1|
AAV Capsid Library(II)
AAV Capsid Libraries(II) are random peptide insert library build on our ATHENA II platform. They are used to evolute novel AAV capsids with tissue-specific tropism. In the premade AAV Capsid library(II), CAP gene with random peptide are driven by a CAG/P40 hybrid promoter. P40 promoter was used to drive CAP expression during AAV production. CAG promoter is used to express CAP in targeting cells or tissues. AAVnerGene provides AAV Capsid libraries(II) with different serotypes, insert variable regions and insert random peptide lengths. Each library has complexity over 1e9. All the AAV viruses were produced at 200 copies/cell condition and purified with two rounds of CsCl ultracentrifuge.
AAV Library Templates
AAV1, AAV2, AAV3B, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAVrh.10, AAVrh.74
Insert Variable Regions
Insert Random Peptide Length
NNK7, NNK8, NNK9, NNK10, NNK11, NNK12
|AAV1 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV10 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV2 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV3B Capsid Libraries||$9,999.00 – $69,999.00|
|AAV4 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV5 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV6 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV7 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV8 Capsid Libraries||$9,999.00 – $69,999.00|
|AAV9 Capsid Libraries||$9,999.00 – $69,999.00|
|AAVrh.10 Capsid Libraries||$9,999.00 – $69,999.00|
|AAVrh74 Capsid Libraries||$9,999.00 – $69,999.00|