AAV Production Kit
AAV Production Kit
AAVnerGene provides integrated AAV production kits designed to simplify and optimize transient AAV manufacturing. The platform combines key components that have been selected and optimized to work together, providing robust cell growth, efficient transfection, and high AAV productivity.
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Kit Components
Each AAV Production Kit includes the key components required for efficient suspension-based AAV vector production:
- HEK293one® Cell Line – 1 vial
- CelliMax™ Culture Medium –2 × 1 L
- PEIone™ Transfection Reagent – Sufficient for up to 1 L of culture
- ENone™ Production Enhancer – Sufficient for up to 1 L of culture
Together, these components provide a ready-to-use, scalable platform for high-yield and reproducible AAV production, from small-scale research applications to process development and manufacturing.
AAV Production Related Products
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HEK293one cell line
HEK293one® is a high-performance suspension HEK293 cell line developed by AAVnerGene specifically for recombinant adeno-associated virus (rAAV) production. The cell line was selected and optimized using the AAVone® production platform to provide robust cell growth, efficient transfection, and high AAV vector productivity. HEK293one® is adapted to serum-free suspension culture and is suitable for scalable AAV manufacturing from shake flasks to bioreactors. When combined with AAVone2.1®, CelliMax™ Medium, PEIone™, and ENone™, HEK293one® provides an integrated platform for high-yield and reproducible AAV production.
Key Features
Selected with AAVone single-plasmid AAV production system
Stable single-cell suspension growth without clump formation
Fully adapted to CelliMax, FreeStyle F17/Viral Production Medium, PEAK/PRIME, and Pro293 media
High AAV productivity across multiple production platforms and compatibility with various AAV serotypes
High full AAV particles compatibility with various AAV serotypes
High transfection efficiency and compatible with multiple transfection reagents, especially our PEIone and ENone
Available for research use under license, cGMP cell banks are currently in preparation
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PEIone™ Transfection Reagent
PEIone™ is a high-performance polyethylenimine (PEI)-based transfection reagent optimized specifically for recombinant AAV production. It delivers efficient plasmid transfection with excellent reproducibility across suspension and adherent HEK293-derived producer cell lines. Designed to support both small-scale research and large-scale manufacturing, PEIone™ provides robust transfection efficiency while maintaining high cell viability, making it an ideal choice for high-yield AAV production.
Key Features
High DNA-binding capacity with PEIone/pDNA=0.7
High transfection efficiency
Exceptional complex stability, ensuring consistent transfection efficiency
Excellent reproducibility between batches
Compatible with HEK293one™, VPC2.0, and other HEK293-derived cell lines
Compatible with single-, dual-, and triple-plasmid production systems
Easily scalable from laboratory to bioreactor production
ENone™ AAV Production Enhancer
ENone™ AAV Enhancer is a proprietary, ready-to-use cell culture supplement that significantly improves recombinant AAV production. Added immediately before or shortly after transfection, ENone™ enhances the cellular environment to promote efficient vector production while maintaining excellent vector quality.
ENone™ has demonstrated consistent performance across multiple AAV serotypes, producer cell lines, culture media, and transfection reagents, making it an easy and effective solution for improving AAV manufacturing.
Key Features
- 2–7× higher AAV vector yield across a broad range of production conditions
- Ready-to-use formulation requiring no preparation
- Simple 1:100 (v/v) addition after transfection
- Compatible with multiple AAV serotypes
- Works with HEK293one and other HEK29 cell lines
- Compatible with PEIone™ and other commonly used transfection reagents
- Easily integrated into existing workflows
ENone™ was added immediately after transfection. Results demonstrate the enhancement in vector yield achieved with ENone™ compared with the no-enhancer control.
CelliMax Suspension Culture Medium
CelliMax™ Suspension Culture Medium is a high-performance, chemically defined (CD) basal medium developed by AAVnerGene for high-density suspension culture of HEK293 cells and efficient viral vector production.
CelliMax™ supports robust cell growth, high viability, and consistent transfection performance across different HEK293 cell types. The medium is particularly optimized for AAV production and delivers its best performance when combined with the HEK293one® cell line, AAVone® production system, PEIone™ transfection reagent, and ENone™ production enhancer.
Key Features
- Chemically defined (CD) formulation
- Designed for high-density HEK293 suspension culture
- Supports robust cell growth and high viability
- Optimized for high-yield AAV vector production
- Compatible with different HEK293 cell lines
- Optimized for HEK293one® cells
- Compatible with AAVone®, PEIone™, and ENone™
- Suitable for multiple AAV serotypes and engineered capsids
- Supports lentiviral vector production
- Suitable for shake-flask and bioreactor scale-up
- Designed for research, process development, and scalable viral vector manufacturing
CelliMax™ provides an optimized culture environment for high-performance HEK293 growth and viral vector production.