pAAVone-sc-AAV1-CMV-EGFP

pAAVone-sc-AAV1-CMV-EGFP is a plasmid used to produce scAAV1-CMV-EGFP using AAVnerGene’s novel AAVone production system. It  integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV1), and a self-complementary AAV-CMV-EGFP genome.  scAAV1-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV1 is a serotype of adeno-associated virus that is known for its ability to efficiently transduce muscle, heart, and certain neuronal tissues. It has been widely used in gene therapy, especially for conditions where skeletal muscle transduction is critical. Compared to other AAV serotypes, such as AAV2, AAV1 has a stronger affinity for muscle tissues, making it a popular choice for muscle-targeted gene therapies.

• AAV Capsid: AAV1
• AAV Genome: sc-AAV-CMV-SV40Intron-EGFP-WPRE3-SV40PolyA
• AAV Genome Size: 2167 bp
• AAV Genome Type: scAAV
• ITRs:  L-ITR (106 bp) + R-ITR (130 bp)
• Promoter: CMV
• Transgene: EGFP
• Plasmid Size: 10641 bp
• Bacterial Resistance(s) : Kana
• Plasmid Backbone: pAAVone-sc
• Storage:  -20°C for long term (> 1 year)
• Shipping: Dry ice.
• Notice to Purchaser:  MTA is required. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
• Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
• AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
• AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.