pAAVone-AAV9-CMV-EGFP-4.6kb
pAAVone-AAV9-CMV-EGFP-4.6kb
pAAVone-AAV9-CMV-EGFP-4.6kb
  1. pAAVone-AAV9-CMV-EGFP-4.6kb
  2. pAAVone-AAV9-CMV-EGFP-4.6kb
  3. pAAVone-AAV9-CMV-EGFP-4.6kb

pAAVone-AAV9-CMV-EGFP-4.6kb

Price range: $999.00 through $1,899.00

 pAAVone-AAV9-CMV-EGFP Series are plasmids used to evaluate the productivity of AAVnerGene’s novel AAVone production system for different size of transgenes. The basic pAAVone-AAV9-CMV-EGFP carries 2.2 kb of AAV-CMV-EGPF genome.  In the pAAVone-AAV9-CMV-EGFP-4.6kb plasmid,  a 2.4 kb of LacZ stuff DNA is  inserted between L-ITR and CMV promoter to make a 4.6 kb final AAV cassette
  • AAV Capsid:  AAV9
  • AAV Genome: AAV-CMV-EGFP-4.6 kb
  • AAV Genome Size: 4,636 bp with 2.4 kb of LacZ as stuff sequence.
  • AAV Genome Type: ssAAV
  • ITRs:  L-ITR (119 bp) + R-ITR (130 bp)
  • Promoter: CMV
  • Transgene: EGFP
  • Plasmid Size: 17,589 bp
  • Bacterial Resistance(s) : Kana
  • Plasmid Backbone: pAAVone
  • Storage:  -20°C for long term (> 1 year)
  • Shipping: Dry ice.
  • Notice to Purchaser:  MTA is required. All mini-pHelper based products, including mini-pHelper itself, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
  • Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
  • AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin).
  • AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.
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SKU: SE001001-4.6kb Categories: , ,

AAVone Productivity with Different Payloads​:

pAAVone names AAV

Genome

 Payload

size(Kb)

 Plasmid

Size(Kb)

 AAV

Serotype

 Productivity

(10^15 VG/L)

pAAVone-AAV9-CMV-EGFP AAV-CMV-EGFP 2.2 15.2 AAV9 2.87 ± 0.18
pAAVone-AAV9-CMV-EGFP-4.0kb AAV-CMV-EGFP-4.0K 4.0 17.0 AAV9 2.36 ± 0.28
pAAVone-AAV9-CMV-EGFP-4.6kb AAV-CMV-EGFP-4.6K 4.6 17.6 AAV9 1.76 ± 0.65
pAAVone-AAV9-CMV-EGFP-4.7kb AAV-CMV-EGFP-4.7K 4.7 17.7 AAV9 1.94 ± 0.02

pAAVone-AAV9-CMV-EGFP with different AAV genome sizes:

NameSKUPriceBuy

Advantages of AAVone system compared with traditional triple plasmid system:

1. Increase AAV yield up to 400%.
2. Reduce plasmid number from 3 to 1.
3.Reduce plasmid amount from 1 to 0.25 ug/1e6 cells.
4.Reduce batch variations.
5.Without need the plasmid ratio optimization steps.
6.Compact plasmid size(16±2kb) and comparable yields.
7.Comparable or increased full-to-empty ratio.
8.Similar viral activity, capsid and genomic components.
9.Similar levels of rcAAV, hcDNAs and producer plasmid contaminations.
10.The same upstream and downstream processes, analytical release or characterization assays.

In summary,  pAAVone-CMV-EGFP series plasmids  offer a simple, low cost, but high consistency and high efficiency for production of AAV-CMV-EGFP virus with different AAV serotypes, especially for large scale and GMP grade AAV production.

 

Workflow of AAVone production system:

Workflow of AAVone System-3

AAVone system increase AAV yield up to 400% compared with traditional triple plasmid system:

AAVone system generated high quality AAV vectors:

 

 

Plasmid Amount

1 mg, 10 µg, 10 mg, 100 µg

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