Name: pAAVone-AAV1-CMV-EGFP-2.2kb -pAAVone - AAVnerGene
SKU: SE001001-AAV1
Availability: InStock

pAAVone-AAV1-CMV-EGFP-2.2kb

pAAVone-AAV1-CMV-EGFP-2.2kb is a pAAVone plasmid used to produce AAV1-CMV-EGFP using AAVnerGene’s novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV1), and a 2.2kb single-strand AAV-CMV-EGFP genome. AAV1-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV1 is a serotype of adeno-associated virus that was originally isolated from rhesus monkeys. AAV1 can efficiently target the CNS, liver, lungs, heart, and muscle tissues, among others.

Full Plasmid Name: pAAVone-AAV1-CMV-SV40I-EGFP-WPRE3-SV40polyA-2.2kb
AAV Capsid: AAV1
Final AAV Genome: AAV-CMV-SV40I-EGFP-WPRE3-SV40polyA, 2208 bp
Corresponding AAV vectors: DP001001-AAV1
AAV Genome Type: ssAAV
ITRs: L-ITR (119 bp) + R-ITR (130 bp)
Promoter: CMV
Transgene: EGFP
Plasmid Size: 15,161 bp
Bacterial Resistance(s) : Kana
Plasmid Backbone: pAAVone
Storage: -20°C for long term (> 1 year)
Shipping: Dry ice.
Notice to Purchaser: MTA is required. All mini-pHelper-1.0 based products, including mini-pHelper-1.0 itself, pAAVdual and pAAVone plasmids, are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (Iodixanol, CsCl, or AAV-X resin).
AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, full/empty ratios, and a variety of comprehensive analyses based on custom requests.

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AAV1 tropism in C57B6 mouse:

Our data revealed that AAV1 shows relatively high RNA expression in the heart and kidney, but relatively high DNA levels in the liver of C57B6 mice following intravenous (IV) injection.

pAAVone Plasmids:

pAAVone plasmids are used to produce AAVs using AAVnerGene’s novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells.

Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB. If you require any other AAV serotypes or variants, please contact us, and we can construct them within 2 weeks.