pAAVone-AAV-PHP.eB-CMV-EGFP-2.8kb

pAAVone-AAV-PHP.eB-CMV-EGFP-2.8kb is a plasmid used to produce AAV-PHP.eB Reference (Full) and AAV-PHP.eB Reference (Byproduct-Empty) using AAVnerGene’s novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV-PHP.eB), and a 2.8kb single-strand AAV-CMV-EGFP genome. AAV-PHP.eB-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAV-PHP.eB is an engineered variant of the AAV9 serotype that was developed to improve the transduction efficiency of the central nervous system (CNS), particularly in mice. It was designed through directed evolution to cross the blood-brain barrier (BBB) with significantly enhanced efficiency compared to the natural AAV9 serotype, making it a valuable tool for gene delivery to the brain and spinal cord.

• Full Plasmid Name: pAAVone-AAV-PHP.eB-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb
• AAV Capsid: AAV-PHP.eB
• Final AAV Genome:  AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb, 2843 bp
• Corresponding AAV vectors: AAV-PHP.eB Reference (Full) and AAV-PHP.eB Reference (Byproduct-Empty)
• AAV Genome Type: ssAAV
• ITRs:  L-ITR (119 bp) + R-ITR (130 bp)
• Promoter: CMV
• Transgene: EGFP
• Plasmid Size: 15,817 bp
• Bacterial Resistance(s) : Kana
• Plasmid Backbone: pAAVone
• Storage:  -20°C for long term (> 1 year)
• Shipping: Dry ice.
• Notice to Purchaser:  MTA is required. All mini-pHelper-1.0 based products, including mini-pHelper-1.0 itself, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
• Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
• AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (Iodixanol, CsCl, or AAV-X resin).
• AAV Analytical Services: We also offer advanced AAV characterization services,  including AAV purity, vector genome identity, ITR integrity, DNA impurities, full/empty ratios, and a variety of comprehensive analyses based on custom requests.