mini-pHelper-AAV4

mini-pHelper-AAV4

Price range: $999.00 through $1,899.00

mini-pHelper-AAV4 is a compact, optimized plasmid designed to produce AAV4 empty particles or AAV4 vectors. It integrates AAV4 Rep and AAV4 Cap genes into our mini-pHelper-1.0 backbone. Thus, it not only provides adenoviral helper functions (E2A, E4orf6, VA RNA), but also AAV helper functions (Rep and AAV4).  When transfected into host cells alone, it facilitates the generation of truly-empty AAV4 particles without any genetic materials inside of capsids. Co-transfection mini-pHelper-AAV4 with pAAVtri-GOI enables high-efficiency production of recombinant AAV4 vectors carrying your gene of interest (GOI).

  • SKU: SM000002-AAV4
  • AAV Serotype: AAV4
  • Plasmid Size: 12,965 bp
  • Related AAV product: 
  • Bacterial Resistance(s) : Kana
  • Storage:  -20°C for long term (> 1 year)
  • Shipping: Dry ice.
  • Notice to Purchaser:  MTA is required. All mini-pHelper based products, including mini-pHelper itself, mini-pHelper-AAVs, pAAVdual and pAAVone plasmids,  are sold under license of US 20240229067. Rights to use these products are limited to non-commercial research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of these products for commercial purposes should be directed to AAVnerGene Inc, 9620 Medical Center Dr, Suite 100, Rockville, MD, 20850. Purchase of these products does not grant rights to : (1) offer the vectors or any derivatives thereof for resale; or (2) to distribute or transfer the vector or any derivative thereof to third parties.
  • Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
  • AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (Iodixanol, CsCl, or AAV-X resin).
  • AAV Analytical Services: We also offer advanced AAV characterization services,  including Full/empty ratios, AAV purity, AAV potency, vector genome identity, ITR integrity, DNA impurities, rcAAV, and a variety of comprehensive analyses based on custom requests.
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SKU: SM000002-AAV4 Categories: , , ,

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Two-plasmid AAV production systems:

By utilizing different combinations, it is possible to create three distinct types of two-plasmid systems for AAV production:

  • Version V1: Combining Ad helper with GOI: V1, exemplified by AAVnerGene’s AAVdual system, stands out for its ability to maintain both packaging efficiency and flexibility. This version allows researchers to work with various AAV serotypes while ensuring efficient AAV vector packaging. This balance is particularly beneficial for projects involving diverse gene therapy or vector biology applications.
  • Version V2: Combining Ad helper with AAV helper: V2, such as the pDG and pQT systems, offers flexibility in producing different AAV serotypes, making it useful for a wide range of research applications. AAVnerGene’s mini-hepler-AAVs are pDG like plasmids, but more compact in size.
  • Version V3: Combining GOI with AAV helper: V3, such as the pOXB system, may provide increased AAV packaging efficiency, which is critical for maximizing the yield of properly packaged AAV vectors. However, it sacrifices flexibility by linking the GOI with the AAV helper plasmid, making it more challenging to switch and customize the GOI.
Two-plasmid AAV Production Systems
Two-plasmid AAV Production Systems
Amount

10 μg, 100 μg, 1 mg, 10 mg

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