AAVrh.74 Reference-2.8kb
AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb
  1. AAVrh.74 Reference-2.8kb
  2. AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb

AAVrh.74 Reference-2.8kb

Price range: $399.00 through $2,799.00

The AAVrh.74 reference-2.8kb material contains a 2.8kb AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA genome in AAVrh.74 capsid, with a genome titer (EGFP targeting) of 1X10^13 VG/ml. This reference is produced using the AAVone system with the pAAVone-AAVrh.74-CMV-EGFP-2.8kb plasmid. AAVrh.74-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAVrh.74 is a AAV serotype derived from rhesus monkeys. It has gained prominence for its ability to efficiently transduce muscle tissue, making it particularly suitable for gene therapy applications targeting muscle disorders such as Duchenne muscular dystrophy (DMD, ELEVIDYS). AAVrh.74 has been shown to have higher transduction efficiency in skeletal and cardiac muscles compared to other AAV serotypes, such as AAV9, and is being used in clinical trials for its potential to deliver therapeutic genes effectively.

  • AAV Serotype: AAVrh.74
  • AAV Genome: AAV-CMV-SV40I-EGFP-WPRE-hGHpA-2.8k
  • AAV Genome Type and Size: ssAAV, 2843 bp, 
  • Promoter: CMV
  • Intron: SV40 Intron
  • Reporter: EGFP
  • Enhancer: WPRE
  • PolyA: hGHpolyA
  • AAV Genome Titer:  1X10^13 VG/ml, targeting EGFP
  • Full particle ratio:  >85% by mass photometry
  • Producer Plasmid: pAAVone-AAVrh.74-CMV-EGFP-2.8kb
  • Producer Cell line: Suspension HEK 293T
  • Purification: AAVx followed by one round of CsCl ultracentrifuge.
  • Endotoxin: < 1.0 EU/ml
  • Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
  • Storage:  -80°C for long term (> 1 year).
  • Shipping: Dry ice.
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SKU: DJ001001-AAVrh.74 Categories: , , ,

AAV References at AAVnerGene:

AAV references are crucial for qPCR-based vector genome quantification and fundamental for ensuring accuracy in viral vector research and gene therapy development. Our AAV reference materials undergo extensive characterization, including full ratio (mass photometry), genome titer (ddPCR), capsid titer (ELISA), purity (SDS-PAGE silver staining), and tests for bioburden, endotoxin, and mycoplasma. Quantification is based on reference material from ATCC. These thoroughly analyzed particles provide precise genome titers, well-defined full-to-empty capsid ratios, and accurate capsid titers. This comprehensive characterization is essential for standardizing measurements, ensuring consistency across experiments, and delivering reliable data for gene therapy applications and other research involving AAV vectors.

AAVnerGene provide three types of AAV references: AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying  AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genomes.

 

AAV References Serotype Genome Production Plasmids
 AAV References-2.8kb AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb
 AAV References-4.3kb AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpA-4.3kb pAAVone-AAVs-CMV-EGFP-4.3kb
AAV References (Truly-Empty) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 No mini-pHelper-AAVs
AAV References (Byproduct-Empty) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 Partial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb

 

AAV references (Full) with different AAV serotypes

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Volume

100 µL, 1000 µL, 200 µL, 500 µL

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