Name: AAV-TBG-EGFP - AAV Viruses - AAVnerGene
SKU: YL006004-AV9
Price: 249.00 USD
Availability: InStock

AAV9-hSyn-ATP1.0mut (B09 N283A)

$249.00

AAV9-hSyn-ATP1.0mut viruses are ready to use AAV vectors carrying hSyn-ATP1.0mut expression cassette. AAVnerGene also offers hSyn-ATP1.0mut vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.

The hSyn promoter, also known as the human Synapsin 1 promoter, is a regulatory sequence used to drive gene expression specifically in neurons. Synapsin 1 is a neuron-specific phosphoprotein that plays a crucial role in the regulation of neurotransmitter release at synapses. The hSyn promoter is a neuron-specific regulatory element that drives strong and selective gene expression in neurons. It is widely used in neuroscience research, gene therapy, the development of transgenic models, and optogenetic studies. Its specificity to neurons makes it invaluable for targeting gene expression in the nervous system, facilitating research into neuronal function, connectivity, and neurological disorders.

ATP1.0mut is an engineered, ATP‑insensitive mutant version of the GRAB ATP1.0 sensor, designed to serve as a negative control in experiments measuring extracellular ATP. It retains the structural framework of the P2Y1‑based cpGFP sensor but contains mutations that prevent ATP‑induced conformational changes, resulting in no fluorescence response upon ATP binding.

AAV Plasmids: pAAV-hSyn-ATP1.0mut
AAV Genomic Type: Single strand AAV (ssAAV)
AAV Gene Specific Titer: 1X10^13 VG/ml
AAV Full Percentage: >85%
AAV Production System: AAVtri system
AAV Production Cells: HEK 293T
AAV Purification: Iodixanol Gradient Ultracentrifugation
Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
Shipping: Dry ice

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AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.

Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:

Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.