Name: AAV-TBG-EGFP - AAV Viruses - AAVnerGene
SKU: YL005008-AV9
Price: 249.00 USD
Availability: InStock

AAV9-GfaABC1D-Ado1.0med

$249.00

AAV9-GfaABC1D-Ado1.0med viruses are ready to use AAV vectors carrying GfaABC1D-Ado1.0 expression cassette. AAVnerGene also offers GfaABC1D-Ado1.0 vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.

The GfaABC1D promoter is a specific regulatory DNA sequence that controls the expression of the GfaABC1D gene. This promoter is typically associated with driving gene expression in glial fibrillary acidic protein (GFAP)-expressing cells, particularly astrocytes in the central nervous system. The GfaABC1D promoter is a shortened version of the longer GFAP promoter, optimized for more specific or efficient expression in certain experimental contexts. This promoter is widely used in gene therapy, neuroscience, and molecular biology to target gene expression specifically to astrocytes. Researchers often use it in experiments where controlling gene expression in these glial cells is crucial, such as in studies of neurodegenerative diseases, brain injury, or astrocyte function.

Compared with the higher‑affinity Ado1.0 variant, Ado1.0med is better suited for detecting larger, physiologically relevant changes in adenosine without saturation, making it useful for studying slow somatodendritic adenosine release revealed in recent in‑vivo work.

AAV Plasmids: pAAV-GfaABC1D-Ado1.0med
AAV Genomic Type: Single strand AAV (ssAAV)
AAV Gene Specific Titer: 1X10^13 VG/ml
AAV Full Percentage: >85%
AAV Production System: AAVtri system
AAV Production Cells: HEK 293T
AAV Purification: Iodixanol Gradient Ultracentrifugation
Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
Shipping: Dry ice

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AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.

Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:

Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.