Name: AAV-TBG-EGFP - AAV Viruses - AAVnerGene
SKU: YL005002-AV9(Cre dependent)
Availability: InStock

AAV9-EFS-DIO-Ado1.0 (B10)

AAV9-EFS-DIO-Ado1.0 viruses are ready to use AAV vectors carrying EFS-DIO-Ado1.0 expression cassette. AAVnerGene also offers EFS-DIO-Ado1.0 vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.

The EFS promoter, a truncated version of the human EF1α regulatory sequence, is engineered to deliver dependable, continuous gene expression in a wide range of cell types while occupying minimal vector space. By condensing the EF1α promoter into a compact format, it preserves strong transcriptional output yet remains far smaller than the full‑length version, making it well suited for applications with limited cargo capacity.

The Ado1.0 is a GPCR‑based EGFP fluorescent sensor designed to visualize extracellular adenosine dynamics with high sensitivity and cellular specificity. It is engineered by integrating a circularly permuted fluorescent protein into the human A2A adenosine receptor so that ligand‑induced structural shifts produce measurable fluorescence changes.

DIO‑Ado1.0 is a Cre‑dependent version of the medium‑affinity GRAB adenosine sensor, engineered so that expression occurs only in cells expressing Cre recombinase. It uses the EFS promoter to drive reliable, constitutive expression once activated, enabling targeted measurement of extracellular adenosine dynamics in defined neuronal populations.

AAV Plasmids:AAV9-EFS-DIO-Ado1.0
AAV Genomic Type: Single strand AAV (ssAAV)
AAV Gene Specific Titer: 1X10^13 VG/ml
AAV Full Percentage: >85%
AAV Production System: AAVtri system
AAV Production Cells: HEK 293T
AAV Purification: Iodixanol Gradient Ultracentrifugation
Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
Shipping: Dry ice

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AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.

Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:

Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.