AAV9-CaMKII-ACh3.0 viruses are ready to use AAV vectors carrying CaMKII-ACh3.0 expression cassette. AAVnerGene also offers CaMKII-ACh3.0 vectors with >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The CaMKIIα promoter refers to the regulatory sequence that controls the expression of the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is an essential enzyme predominantly expressed in the brain, especially in excitatory neurons of the forebrain, such as those found in the hippocampus and cortex. The CaMKIIα promoter is a neuron-specific regulatory element that drives gene expression in excitatory neurons of the forebrain, particularly in regions like the hippocampus and cortex. It is a critical tool in neuroscience research, transgenic model development, optogenetics, and potentially gene therapy. Its specificity to neurons involved in synaptic plasticity, learning, and memory makes it invaluable for studying the molecular and cellular mechanisms underlying these key brain functions.
ACh3.0 is an improved genetically encoded EGFP fluorescent acetylcholine sensor from the GRAB family, engineered from the earlier ACh2.0 design to provide significantly higher sensitivity and lower downstream GPCR signaling. It is built using the human M3 muscarinic acetylcholine receptor with a cpEGFP inserted into its intracellular loop, allowing real‑time visualization of endogenous ACh dynamics in vivo. Compared to ACh2.0, ACh3.0 offers enhanced signal amplitude and greatly reduced coupling to endogenous signaling pathways, enabling more accurate monitoring of cholinergic activity.
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. To learn more about biosensors, feel free to refer our blog Fluorescent Biosensors for Neurotransmission and Neuromodulation.
Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
Full/Empty Particle Ratio: Ensure all the premade AAVs has >85% full particles by Mass Photometry.
Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
SDS-PAGEGel: Assess the purity of the AAV viruses is great than 95%.
Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
$999.00–$1,899.00Price range: $999.00 through $1,899.00Select options
This product has multiple variants. The options may be chosen on the product page
$399.00–$2,799.00Price range: $399.00 through $2,799.00Select options
This product has multiple variants. The options may be chosen on the product page
$399.00–$2,799.00Price range: $399.00 through $2,799.00Select options
This product has multiple variants. The options may be chosen on the product page
$399.00–$2,799.00Price range: $399.00 through $2,799.00Select options
This product has multiple variants. The options may be chosen on the product page
