AAV Reference (Truely-Empty)
AAV6 Reference (Truly-Empty)
AAV6 Reference (Truly-Empty)
AAV6 Reference (Truly-Empty)
AAV Purity
  1. AAV Reference (Truely-Empty)
  2. AAV6 Reference (Truly-Empty)
  3. AAV6 Reference (Truly-Empty)
  4. AAV6 Reference (Truly-Empty)
  5. AAV Purity

AAV6 Reference (Truly-Empty)

Price range: $299.00 through $1,799.00

AAV6 Reference (Truly-empty) particles composed of the same viral proteins (VP1, VP2, and VP3) as regular AAV6 vectors but devoid of any genomic materials, which is different with AAV byproduct-empty particles. This AAV6 truly-empty particles are generated by using our mini-pHelper-AAV6 single-plasmid transfection. AAV truly-empty particles serve as valuable tools for various stages of gene therapy development and characterization.

  • SKU: DE000000-AAV6
  • AAV Serotype: AAV6
  • AAV Capsid titer: 1.0×1013 VP/ml (VP: Viral Particle) by Elisa
  • Used cell line: HEK 293T
  • Used plasmid: mini-pHelper-AAV6
  • Purification method: AAVx resin
  • Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
  • Endotoxin: <1U/1.0×1013 VP
  • Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
  • Shipping: Dry ice.
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SKU: DE000000-AAV6 Categories: , Tag:

AAV References at AAVnerGene:

AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying a 2.8 kb AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genome. AAV reference materials are available for various serotypes, including AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV6, and AAVrh.74. AAV references with other serotypes and genomes are available upon request.

AAV References Serotype Genome Production Plasmids
 AAV References (Full) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV6, AAVrh.74 Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb
AAV References (Truly-Empty) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV6, AAVrh.74 No mini-pHelper-AAVs
AAV References (Byproduct-Empty) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV6, AAVrh.74 Partial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb

AAV reference (Truly-Empty) with different AAV serotypes

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Usage of Empty AAV particles: 

Truly empty capsids devoid of any genomic materials. They serve as valuable tools for various stages of gene therapy development and characterization.

  1. Reference Materials: Well-characterized empty AAV capsids, can serve as standardized reference materials for analytical assays.
  2. Quality Control: During the manufacturing process of AAV vectors, using well-characterized capsids as reference materials allows manufacturers to monitor and ensure the quality, purity, and consistency of their AAV products.
  3. Biodistribution Studies: Empty AAV can be used in biodistribution studies to assess how different capsids distribute in various tissues after administration.
  4. Immunogenicity Assessment: By exposing the immune system to well-characterized AAV capsids, researchers can evaluate the immune responses induced by specific serotypes..
  5. Comparative Studies: Empty AAV allow researchers to compare the characteristics of different AAV serotypes without the confounding effects of transgene expression.
  6. Process Development: Empty AAV can be used in the development and optimization of manufacturing processes for AAV vectors.
  7. Assay Development: Empty AAV capsids serve as positive controls in various assays, aiding the development of new methods for AAV characterization, stability testing, and potency determination.
  8. Gene Therapy Capsid Decoys: The presence of an excess of empty AAV capsids may effectively absorb low-level neutralizing antibodies (NAbs) and non-NAbs, permitting transduction even in their presence.
Volume

100 µL, 200 µL, 500 µL, 1000 µL

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