AAV6 Capsid Libraries
Price range: $9,999.00 through $69,999.00
AAV6 Capsid Libraries are designed for the evolution and development of novel AAV capsids with specific characteristics based on AAV6 capsid. AAV6 Capsid Libraries have 7 to 12 amino acid random peptides insertion on the surface of viral capsid at VR-IV or VR-VIII region. AAV6 CAP gene with random peptide are driven by a CAG/P40 hybrid promoter. P40 promoter was used to drive CAP expression during AAV production. CAG promoter is used to express CAP in targeting cells or tissues.
- SKU: DB005-AAV6
- AAV Capsid Library: ATHENA-II random peptide insertion
- Library Titer: 1e13 VG/ml
- Promoter: CAG/P40
- AAV serotype: AAV6
- Insert Region(site): VR-IV(G454_S455) or VR-VIII(Q585_S586)
- Insert Random Peptide Length: 7-12 amino acids
- Complexity: >1e9
- Production Condition: 200 copies/cell
- Production Cell Line: HEK 293T
- Purification Method: Two rounds of CsCl ultracentrifuge
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks.
- Shipping: Dry ice.
AAVnerGene’s AAV capsid libraries are random peptide insert library build on our ATHENA-II platform. They are used to evolute novel AAV capsids for different purposes. Each library has high library complexity which provides a huge number of screening resources for AAV capsid directed evolution study. With our novel library construction strategies, we offer generation of high complexity, random peptide insertion AAV capsid libraries at any site and at any peptide length in addition to the libraries listed in the catalog.
In the premade AAV Capsid library, CAP gene with random peptide are driven by a CAG/P40 hybrid promoter. P40 promoter was used to drive CAP expression during AAV production. CAG promoter is used to express CAP in targeting cells or tissues. In the premade AAV capsid libraries, each library has complexity over 1e9 and has titer over 1e13 GC/ml. AAV viral libraries were produced at 200 copies/cell conditions in HEK 293T cells and purified with two rounds of CsCl ultracentrifuge.
Highlights:
- Ready-to-use
- Multiple insert regions and insert lengths
- High complexity: >1e9
- Low cross-packaging and capsid mosaicism
- Low stop codon ratio
- Low backbone contamination
- High purity
AAV capsid libraries with other serotypes:
| Name | SKU | Price | Buy |
|---|
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| Insert Region | VR-IV, VR-VIII |
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