AAV5 Reference-4.3kb
Price range: $399.00 through $2,799.00
The AAV5 reference-4.3kb material contains a 4.3kb AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA genome (with a 1.5kb LacZ) in AAV5 capsid, with a genome titer (EGFP targeting) of 1X10^13 VG/ml. AAV5 is a serotype of adeno-associated virus that was originally isolated from rhesus monkeys, with unique tissue tropism and characteristics that make it useful in specific gene therapy applications. AAV5 has demonstrated an ability to transduce various tissues, including the brain, lungs, and liver. Gene therapy based on AAV5 has been approved for human use, such as Roctavian (valoctocogene roxaparvovec), developed by BioMarin Pharmaceutical for the treatment of hemophilia A.
- AAV Serotype: AAV5
- AAV Genome: AAV-CMV-SV40I-EGFP-WPRE-hGHpA-4.3kb
- AAV Genome Type and Size: ssAAV, 4341 bp,
- Promoter: CMV
- Intron: SV40 Intron
- Reporter: EGFP
- Enhancer: WPRE
- PolyA: hGHpolyA
- AAV Genome Titer: 1X10^13 VG/ml, targeting EGFP
- Full particle ratio: >85% by mass photometry
- Producer Cell line: Suspension HEK 293T
- Purification: AAVx followed by one round of CsCl ultracentrifuge.
- Endotoxin: < 1.0 EU/ml
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
- Storage: -80°C for long term (> 1 year).
- Shipping: Dry ice.
AAV References at AAVnerGene:
AAV references are crucial for qPCR-based vector genome quantification and fundamental for ensuring accuracy in viral vector research and gene therapy development. Our AAV reference materials undergo extensive characterization, including full ratio (mass photometry), genome titer (ddPCR), capsid titer (ELISA), purity (SDS-PAGE silver staining), and tests for bioburden, endotoxin, and mycoplasma. Quantification is based on reference material from ATCC. These thoroughly analyzed particles provide precise genome titers, well-defined full-to-empty capsid ratios, and accurate capsid titers. This comprehensive characterization is essential for standardizing measurements, ensuring consistency across experiments, and delivering reliable data for gene therapy applications and other research involving AAV vectors.
AAVnerGene provide three types of AAV references: AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genomes.
| AAV References | Serotype | Genome | Production Plasmids |
| AAV References-2.8kb | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb | pAAVone-AAVs-CMV-EGFP-2.8kb |
| AAV References-4.3kb | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpA-4.3kb | pAAVone-AAVs-CMV-EGFP-4.3kb |
| AAV References (Truly-Empty) | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | No | mini-pHelper-AAVs |
| AAV References (Byproduct-Empty) | AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, AAVrh.74 | Partial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb | pAAVone-AAVs-CMV-EGFP-2.8kb |
AAV references (Full) with different AAV serotypes
| Name | SKU | Price | Buy |
|---|
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
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