AAV-K14-EGFP
Price range: $299.00 through $1,799.00
AAV-K14-EGFP series viruses are ready to use AAV vectors carrying K14-EGFP expression cassette. AAVnerGene offers AAV-K14-EGFP vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The K14 promoter is an epithelial-specific regulatory element that drives gene expression in the basal layer of the epidermis and other stratified epithelia. It is a critical tool in skin and epithelial research, gene therapy for skin disorders, and the creation of transgenic models to study skin development and disease. Its specificity to basal epithelial cells makes it invaluable for targeting gene expression in the skin and related tissues, particularly in the context of studying and treating epithelial diseases.
By employing EGFP as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.
- AAV Plasmids: pAAVdual-K14-EGFP + pRCap-AAVs
- AAV Genome: AAV-K14-SV40intron-EGFP-WPRE3-SV40polyA
- AAV Genomic Type: Single strand AAV (ssAAV)
- AAV Genomic Size: 3900 bp
- AAV Gene Specific Titer: 1X10^13 VG/ml
- AAV Full Percentage: >85%
- AAV Production System: AAVdual system
- AAV Production Cells: HEK 293T
- AAV Purification: Two rounds of CsCl ultracentrifuge
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
- Shipping: Dry ice
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| AAV Serotype | AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74 |
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