AAV-Iba1-EGFP
Price range: $299.00 through $1,799.00
AAV-Iba1-EGFP series viruses are ready to use AAV vectors carrying Iba1-EGFP expression cassette. The Iba1 promoter refers to the regulatory sequence that controls the expression of the Ionized calcium-binding adaptor molecule 1 (Iba1) gene. Iba1 is a protein highly expressed in microglia, the resident immune cells of the central nervous system (CNS), as well as in macrophages. The Iba1 promoter is a microglia- and macrophage-specific regulatory element that drives gene expression in these immune cells, particularly within the CNS. It is an essential tool in neuroscience research, gene therapy for CNS diseases, and the development of transgenic models to study microglia and macrophage function. Its specificity to microglia makes it invaluable for targeting gene expression in studies focused on neuroinflammation, neurodegeneration, and CNS immune responses. By employing EGFP as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.
AAVnerGene offers AAV-Iba1-EGFP vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.
- Plasmids: pAAVdual-Iba1-EGFP + pRCap-AAVs
- AAV Genome: AAV-GfaABC1D-SV40I-EGFP-WPRE3-SV40polyA.
- AAV Genomic Type: Single strand AAV (ssAAV).
- AAV Size: 2350 bp.
- AAV Titer: 1X10^13 VG/ml.
- AAV Full Particle Ratio: >85%.
- AAV Production System: AAVdual system.
- AAV Production Cells: HEK 293T.
- AAV Purification: Two rounds of CsCl ultracentrifuge.
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks.
- Shipping: Dry ice
- Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone.
- AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (Iodixanol, CsCl, or AAV-X resin).
- AAV Analytical Services: We also offer advanced AAV characterization services, including full/empty ratios, AAV purity, AAV potency, vector genome identity, ITR integrity, DNA impurities, rcAAV, and a variety of comprehensive analyses based on custom requests.
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| AAV Serotype | AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74 |
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