AAV-EF1a-iCre
Price range: $299.00 through $1,799.00
AAV-EF1a-iCre series viruses are ready to use AAV vectors carrying EF1a-iCre expression cassette. AAVnerGene offers AAV-EF1a-iCre vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.
The EF1a promoter refers to the promoter region of the Elongation Factor 1-alpha (EF1a) gene, which is responsible for driving the expression of EF1a, a protein that plays a key role in the elongation phase of protein synthesis. The EF1a promoter is a strong, ubiquitous promoter that drives constitutive gene expression across a wide range of cell types. It is widely used in gene therapy, transgenic model creation, protein production, and research on gene function. Its broad activity and ability to drive high-level expression make it a valuable tool for experiments requiring robust and consistent gene expression in diverse biological systems.
iCre is a codon-optimized variant of Cre recombinase, designed to enhance its expression and efficiency in mammalian cells. Like the standard Cre recombinase, iCre recognizes loxP sites and catalyzes site-specific recombination between them, which can result in the deletion, inversion, or translocation of the DNA sequence flanked by these sites. AAV-iCre offers an improved and more efficient approach to conditional gene manipulation, providing researchers with a powerful tool for studying gene function, lineage tracing, and gene editing.
- AAV Plasmids: pAAVdual-EF1a-iCre + pRCaps
- AAV Genome: AAV-EF1a-SV40intron-iCre-WPRE3-SV40polyA
- AAV Genomic Type: Single strand AAV (ssAAV)
- AAV Genomic Size: 3150 bp
- AAV Gene Specific Titer: 1X10^13 VG/ml
- AAV Full Percentage: >85%
- AAV Production System: AAVdual system
- AAV Production Cells: HEK 293T
- AAV Purification: Two rounds of CsCl ultracentrifuge
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
- Shipping: Dry ice
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| AAV Serotype | AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74 |
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