AAV Reference (Truely-Empty)
AAV-DJ Reference (Truly-Empty)
AAV-DJ Reference (Truly-Empty)
AAV-DJ Reference (Truly-Empty)
AAV Purity
  1. AAV Reference (Truely-Empty)
  2. AAV-DJ Reference (Truly-Empty)
  3. AAV-DJ Reference (Truly-Empty)
  4. AAV-DJ Reference (Truly-Empty)
  5. AAV Purity

AAV-DJ Reference (Truly-Empty)

Price range: $299.00 through $1,799.00

AAV-DJ Reference (Truly-empty) particles composed of the same viral proteins (VP1, VP2, and VP3) as regular AAV-DJ vectors but devoid of any genomic materials, which is different with AAV byproduct-empty particles. This AAV-DJ truly-empty particles are generated by using our mini-pHelper-AAV-DJ single-plasmid transfection. AAV truly-empty particles serve as valuable tools for various stages of gene therapy development and characterization.

  • SKU: DE000000-AAV-DJ
  • AAV Serotype: AAV-DJ
  • AAV Capsid titer: 1.0×1013 VP/ml (VP: Viral Particle) by Elisa
  • Used cell line: HEK 293T
  • Used plasmid: mini-pHelper-AAV-DJ
  • Purification method: AAVx resin
  • Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
  • Endotoxin: <1U/1.0×1013 VP
  • Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
  • Shipping: Dry ice.
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SKU: DE000000-AAV-DJ Categories: , Tag:

AAV References at AAVnerGene:

AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying a 2.8 kb AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genome. AAV reference materials are available for various serotypes, including AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ, and AAVrh.74. AAV references with other serotypes and genomes are available upon request.

AAV References Serotype Genome Production Plasmids
 AAV References (Full) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAVrh.74 Full of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb
AAV References (Truly-Empty) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAVrh.74 No mini-pHelper-AAVs
AAV References (Byproduct-Empty) AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAVrh.74 Partial of AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb pAAVone-AAVs-CMV-EGFP-2.8kb

AAV reference (Truly-Empty) with different AAV serotypes

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Usage of Empty AAV particles: 

Truly empty capsids devoid of any genomic materials. They serve as valuable tools for various stages of gene therapy development and characterization.

  1. Reference Materials: Well-characterized empty AAV capsids, can serve as standardized reference materials for analytical assays.
  2. Quality Control: During the manufacturing process of AAV vectors, using well-characterized capsids as reference materials allows manufacturers to monitor and ensure the quality, purity, and consistency of their AAV products.
  3. Biodistribution Studies: Empty AAV can be used in biodistribution studies to assess how different capsids distribute in various tissues after administration.
  4. Immunogenicity Assessment: By exposing the immune system to well-characterized AAV capsids, researchers can evaluate the immune responses induced by specific serotypes..
  5. Comparative Studies: Empty AAV allow researchers to compare the characteristics of different AAV serotypes without the confounding effects of transgene expression.
  6. Process Development: Empty AAV can be used in the development and optimization of manufacturing processes for AAV vectors.
  7. Assay Development: Empty AAV capsids serve as positive controls in various assays, aiding the development of new methods for AAV characterization, stability testing, and potency determination.
  8. Gene Therapy Capsid Decoys: The presence of an excess of empty AAV capsids may effectively absorb low-level neutralizing antibodies (NAbs) and non-NAbs, permitting transduction even in their presence.
Volume

100 µL, 200 µL, 500 µL, 1000 µL

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