AAV Capsid Barcode Kit-Retina

AAV Capsid Barcode Kits-Tissues

Tissue-specific AAV capsid Barcode Kits are designed to systematically evaluate AAV capsid variants that have been engineered to exhibit enhanced tropism for specific target tissues. It also includes 15 commonly used AAV serotypes that serve as controls, which are essential for benchmarking and comparative purposes.

Image	Name	SKU	Tags	Price	BuySelect all	hf:tags

AAV Capsid Barcode Kits-Common

Common AAV Capsids: AAV1, AAV2, AAV3B,  AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV-DJ, AAV2-Retro

Image	Name	SKU	Price	BuySelect all

 

Design of AAV Capsid Barcode Kits

AAV Capsid Barcode Kits are known AAV capsid selection kits build on our ATHENA I platform. In the ATHENA I, each capsid variant is associated with three different DNA-barcoded genomes, and all three of these genomes carry the same reporter gene. The use of three DNA barcodes for one capsid variant can minimize experimental variations and improve the accuracy of the results in high-throughput screening or selection processes.

In the AAV Capsid Barcode Kits,  AAV genomes carried different  promoters and different reporter genes, such as AAV-CAG-EGFP, AAV-CMV-mCherry, and scAAV-CMV-mCherry. The presence of the reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes.

The unique barcodes  are located before Poly signal. NGS technology is used to analyze the barcode data after AAV infection or injection.  Researchers can use the unique DNA barcodes to evaluate the distribution of each capsid variant for different types of target cells. This screening method helps identify which AAV capsids are most effective at transducing specific cell types. In addition to DNA barcode screening, transcribed RNA barcodes can be evaluated to assess the potential of different capsids for each target cell. This approach provides insights into the transcriptional activity of the AAV genomes in the context of specific cell types.

Common AAV Capsid List: AAV1, AAV2, AAV3B,  AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV-DJ, AAV2-Retro

 

 

Example ​1:

AAV Capsid Kit: AAV Capsid Barcode Kit-Common-CAG-EGFP

Animal model: C57B6

Injection Route:  IV injection

Time Point: 2 weeks post injection

Analysis method: NGS for BCs

Results: Fold enrichment compared with input AAV viruses

 

Referrences:

1.Immunogenicity of Novel AAV Capsids for Retinal Gene Therapy. Gehrke M, Diedrichs-Möhring M, Bogedein J, Büning H, Michalakis S, Wildner G.Cells. 2022 Jun 9;11(12):1881. doi: 10.3390/cells11121881.

2.Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter. Cronin T, Vandenberghe LH, Hantz P, Juttner J, Reimann A, Kacsó AE, Huckfeldt RM, Busskamp V, Kohler H, Lagali PS, Roska B, Bennett J.EMBO Mol Med. 2014 Sep;6(9):1175-90. doi: 10.15252/emmm.201404077.

3.Targeting ON-bipolar cells by AAV gene therapy stably reverses LRIT3-congenital stationary night blindness Keiko Miyadera, Evelyn Santana, Karolina Roszak, Sommer Iffrig, Meike Visel, Simone Iwabe, Ryan F. Boyd, Joshua T. Bartoe, Yu Sato, Alexa Gray, Ana Ripolles-Garcia, Valérie L. Dufour, Leah C. Byrne, John G. Flannery, William A. Beltran, Gustavo D. Aguirre. Proc Natl Acad Sci U S A. 2022 Mar 29; 119(13): e2117038119. Published online 2022 Mar 22. doi: 10.1073/pnas.2117038119

4.A Novel Adeno-Associated Viral Variant for Efficient and Selective Intravitreal Transduction of Rat Müller Cells. Ryan R. Klimczak, James T. Koerber, Deniz Dalkara, John G. Flannery, David V. Schaffer PLoS One. 2009; 4(10): e7467. Published online 2009 Oct 14. doi: 10.1371/journal.pone.0007467

5.In vivo-directed evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery from the vitreous. Dalkara D, Byrne LC, Klimczak RR, Visel M, Yin L, Merigan WH, Flannery JG, Schaffer DV.Sci Transl Med. 2013 Jun 12;5(189):189ra76. doi: 10.1126/scitranslmed.3005708.

6.High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Petrs-Silva H, Dinculescu A, Li Q, Min SH, Chiodo V, Pang JJ, Zhong L, Zolotukhin S, Srivastava A, Lewin AS, Hauswirth WW.Mol Ther. 2009 Mar;17(3):463-71. doi: 10.1038/mt.2008.269. Epub 2008 Dec 16.