AAV Capsid Barcode Kit-Common-sc-CMV-mCherry
Price range: $5,999.00 through $29,999.00
AAV Capsid Barcode Kit-Common is designed to systematically evaluate different common used AAV serotypes or variants of their activities in different cells and tissues. This library includes 15 common AAV capsids. Researchers can use the unique DNA barcodes to evaluate the distribution of each capsid variant for different types of target cells.
In the AAV Capsid Barcode Kit-Common-scCMV-mCherry, the scAAV-CAG-EGFP genome is used as reporter system. scAAV genome can result rapid AAV-mediated transgene expression in mammalian cells and animals. The CMV promoter is known for strong and ubiquitous expression in various cell types. The presence of the mCherry reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes. WPRE enhancer and bGH polyA can enhance mCherry expression and stabilize mRNA. In this kit, each AAV capsid is assigned to 3 different barcodes (BCs), which are located between WPRE and PolyA. Researchers can check both DNA and RNA levels with NGS targeting to BC region.
- SKU:DA023006
- AAV Expression Cassette: scAAV-CMV-mCherry
- AAV Capsids: AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, AAV2-Retro.
- AAV deliver packaging: Mixed viruses.
- AAV Titer: ~1X10^13 VG/ml.
- BC design: N25
- Production: One AAV capsid and assigned 3 barcode plasmids were co-transfected to make one specific capsid AAV vector using HEK 293T cells.
- Purification: Each AAV capsid were purified with two rounds of CsCl ultracentrifuge and titration with qPCR.
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks.
- Shipping: Dry ice.
AAV capsid Barcode Kits with other reporters
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Tissue-specific AAV Capsid Barcode Kits
Tissue-specific AAV capsid Barcode Kits are designed to systematically evaluate AAV capsid variants that have been engineered to exhibit enhanced tropism for specific target tissues. It also includes 15 commonly used AAV serotypes that serve as controls, which are essential for benchmarking and comparative purposes.
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Design of AAV Capsid Barcode Kits
AAV Capsid Barcode Kits are known AAV capsid selection kits build on our ATHENA I platform. In the ATHENA I, each capsid variant is associated with three different DNA-barcoded genomes, and all three of these genomes carry the same reporter gene. The use of three DNA barcodes for one capsid variant can minimize experimental variations and improve the accuracy of the results in high-throughput screening or selection processes.
In the AAV Capsid Barcode Kits, AAV genomes carried different promoters and different reporter genes, such as AAV-CAG-EGFP, AAV-CMV-EGFP and AAV-CMV-mCherry. The presence of the reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes.
The unique barcodes are located before Poly-A signal. NGS technology is used to analyze the barcode data after AAV infection. Researchers can use the unique DNA barcodes to evaluate the distribution of each capsid variant for different types of target cells. This screening method helps identify which AAV capsids are most effective at transducing specific cell types. In addition to DNA barcode screening, transcribed RNA barcodes can be evaluated to assess the potential of different capsids for each target cell. This approach provides insights into the transcriptional activity of the AAV genomes in the context of specific cell types.
Common AAV Capsid List: AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh.74, AAV-DJ, AAV2-Retro
Example 1:
AAV Capsid Kit: AAV Capsid Barcode Kit-Common-CAG-EGFP
Animal model: C57B6
Injection Route: IV injection
Time Point: 2 weeks post injection
Analysis method: NGS for BCs
Results: Fold enrichment compared with input AAV viruses
| AAV Scale | 1X10^12 VG/Capsid, 2X10^12 VG/Capsid, 5X10^12 VG/Capsid, 10X10^12 VG/Capsid |
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