AAV-CaMKIIα-Cre
Price range: $299.00 through $1,799.00
AAV-CaMKIIα-Cre series viruses are ready to use AAV vectors carrying CaMKIIα-Cre expression cassette. AAVnerGene offers AAV-CaMKIIα-Cre vectors with 16 different serotypes and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.
CaMKIIα promoter refers to the regulatory sequence that controls the expression of the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is an essential enzyme predominantly expressed in the brain, especially in excitatory neurons of the forebrain, such as those found in the hippocampus and cortex. The CaMKIIα promoter is a neuron-specific regulatory element that drives gene expression in excitatory neurons of the forebrain, particularly in regions like the hippocampus and cortex. It is a critical tool in neuroscience research, transgenic model development, optogenetics, and potentially gene therapy. Its specificity to neurons involved in synaptic plasticity, learning, and memory makes it invaluable for studying the molecular and cellular mechanisms underlying these key brain functions.
Cre recombinase is a site-specific DNA recombinase enzyme derived from the bacteriophage P1. Cre recombinase recognizes specific DNA sequences known as loxP sites and catalyzes the recombination between them. This recombination event can lead to the deletion, inversion, or translocation of the DNA segment flanked by loxP sites, depending on the orientation of the sites. AAV-Cre is a powerful tool for genetic research, enabling targeted manipulation of genes in specific tissues or at specific times. Its ability to drive site-specific recombination makes it an essential component of many gene editing, knockout, and lineage tracing studies.
- AAV Plasmids: pAAVdual-CaMKIIα-Cre + pRCaps
- AAV Genome: AAV-CaMKIIα-SV40intron-Cre-WPRE3-SV40polyA
- AAV Genomic Type: Single strand AAV (ssAAV)
- AAV Genomic Size: 3254 bp
- AAV Gene Specific Titer: 1X10^13 VG/ml
- AAV Full Percentage: >85%
- AAV Production System: AAVdual system
- AAV Production Cells: HEK 293T
- AAV Purification: Two rounds of CsCl ultracentrifuge
- Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl
- Storage: -80°C for long term (> 1 year); -20°C for short term(1-2 months); 4 °C for 1-2 weeks
- Shipping: Dry ice
AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses in AAVnerGene are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.
To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their AAV vectors:
- Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
- Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
- Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
- SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
- Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.
| Volume | 100 µL, 1000 µL, 200 µL, 500 µL |
|---|---|
| AAV Serotype | AAV-DJ, AAV-PHP.eB, AAV1, AAV11, AAV12, AAV13, AAV2, AAV2-Retro, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh.74 |
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