AAV-ACTOne-GPCR-Assay-Kit-3.png

AAV ACTOne Gi-GPCR Assay Kit-OPRD1

Price range: $498.00 through $3,598.00

The AAV ACTOne Gi-GPCR Assay Kit-OPRD1 is designed specifically for screening drugs/compounds that target the Delta opioid receptor  (OPRD1) by combination AAV and ACTOne technologies.
The Delta Opioid Receptor (OPRD1) is a G protein-coupled receptor (GPCR) that is part of the opioid receptor family, which also includes the mu (MOR) and kappa (KOR) opioid receptors. OPRD1 is primarily involved in modulating pain perception, mood, and certain immune responses. When activated by its endogenous ligands—such as enkephalins, which are naturally occurring peptides—the receptor signals through the Gi/Go class of G proteins. This activation leads to the inhibition of adenylate cyclase, resulting in decreased cyclic AMP (cAMP) levels and altered neuronal excitability. OPRD1 is expressed in various regions of the central nervous system, including the brain and spinal cord, where it plays a key role in modulating analgesia, emotional responses, and motor function. Delta opioid receptors are known for their ability to produce analgesia with potentially fewer side effects, such as respiratory depression, compared to mu opioid receptors, making them a focus of research for developing safer pain medications. In addition to pain modulation, OPRD1 is involved in the regulation of mood and has been implicated in conditions such as anxiety, depression, and addiction. Agonists and antagonists of the delta opioid receptor are being explored for their therapeutic potential in treating these disorders, as well as for their ability to modulate immune function. Research into OPRD1 continues to explore its diverse roles in the body, with the aim of developing new therapies that leverage its unique properties for pain relief, mood regulation, and other clinical applications.
This kit uses AAV vectors with a CMV promoter to co-express the OPRD1 and cyclic nucleotide-gated (CNG) channel, allowing researchers to conduct high-throughput screening and functional analysis of potential OPRD1-targeting compounds. The kit provides a sensitive and reliable method for evaluating the pharmacological properties of OPRD1 drugs, such as agonists and antagonists, in a live-cell environment.
  • SKU: KI001-OPRD1
  • Targeting GPCR: Human Delta opioid receptor  (OPRD1)
  • GPCR Type: Gi-GPCR
  • Kit components: AAV-CNG/AAV-OPRD1 and ACTOne MP Dye
  • Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
  • Shipping: Dry ice.
Add to Quote
SKU: KI001-OPRD1 Categories: , ,
The AAV ACTOne GPCR Assay Kits are live-cell-based cAMP biosensor systems designed for rapid and high-throughput functional screening of G-protein coupled receptors (GPCRs) by combination AAV. Kits are available for both Gi-GPCR and Gs-GPCR assays.

AAV ACTOne Gi-GPCR Assay Kits:

NameSKUPriceBuy

AAV ACTOne Gs-GPCR Assay Kits:

NameSKUPriceBuy

Advantages of AAV ACTOne GPCR Assay Kits:

  • Combined ACTOne and AAV Technologies
  • Transient Expression of CNG and GPCR
  • Real Time Assay on Living Cell
  • High Sensitivity and Big assay window
  • Both Kinetic and Endpoint Assay
  • Easy to use by mixing the AAVs with cells
  • Faster setup and time efficiency
  • Reduced risk of genetic drift, 
  • Simplified workflows, 
  • flexibility in dynamic control of expression 
  • Low Cost: $498 for one plate
AAV ACTOne GPCR Assay Kit
AAV ACTOne GPCR Assay Kit

ACTOne GPCR Assay

ACTOne™ is the only high-throughput GPCR screening technology that can directly measure the intracellular changes of the secondary messenger cyclic AMP (cAMP) in living cells, in real-time. It uses a proprietary modified cyclic nucleotide-gated (CNG) channel, which is co-localized with adenylate cyclase at the plasma membrane, as a biosensor of cAMP activity. The CNG channel opens when the cAMP level near the plasma membrane increases, resulting in ion flux and cell membrane depolarization. The influx of cations through the CNG channel can be quantified using fluorescent ion indicators or membrane potential (MP) dyes. It provides information on real time intracellular cAMP changes and is highly sensitive. By combining kinetic and endpoint readouts, we are able to capture and analyze transient responses from endogenous GPCRs and weak responses caused by weak Gs or Gi coupled GPCR activities. Using ACTOne, we are able to detect the subcellular cAMP concentration changes directly caused by GPCR activation. Real-time kinetic readouts minimize artifacts, and provide greater content and more statistically relevant data. The intensity of signal increase caused by GPCR activation is directly related to the receptor number on cell surface. Using ACTOne assay, we were able to detect activities of some endogenous Gs coupled receptors in HEK293 cells that have not been reported in literature. In addition, we have also detected weak Gs coupled activity of a GPCR that was widely considered to be only linked to Gq coupled pathway.  The ACTOne assay also provides a useful tool for GPCR de-orphanization.

ACTOne GPCR Assay
ACTOne GPCR Assay
Plate

1-Plate, 2-Plate, 5-Plate, 10-Plate

Related products