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AAV ACTOne Gi-GPCR Assay Kit-NPY2R

Price range: $498.00 through $3,598.00

The AAV ACTOne Gi-GPCR Assay Kit-NPY2R is designed specifically for screening drugs/compounds that target the Neuropeptide Y Receptor Y2 (NPY2R) by combination AAV and ACTOne technologies.
Neuropeptide Y Receptor Y2 (NPY2R) is a G protein-coupled receptor (GPCR) that binds to neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP). The receptor is primarily coupled with the Gi/Go class of G proteins, which inhibit adenylate cyclase, leading to a reduction in cyclic AMP (cAMP) levels. This signaling pathway plays a crucial role in regulating various physiological processes, including appetite suppression, anxiety, and circadian rhythms. NPY2R is predominantly expressed in the central nervous system, particularly in the hypothalamus, where it is involved in the regulation of energy homeostasis and food intake. Unlike the NPY Y1 receptor, which promotes feeding, the activation of NPY2R typically results in the inhibition of food intake, acting as a satiety signal. This makes NPY2R a key player in the control of appetite and body weight regulation. In addition to its role in feeding behavior, NPY2R is also involved in modulating stress responses, anxiety, and neuroendocrine functions. Due to its involvement in these processes, NPY2R is a target of interest for developing therapies aimed at treating obesity, eating disorders, and mood-related conditions. Research into NPY2R continues to focus on its potential as a therapeutic target for modulating appetite and energy balance, as well as its broader role in managing stress and anxiety-related disorders.
This kit uses AAV vectors with a CMV promoter to co-express the NPY2R and cyclic nucleotide-gated (CNG) channel, allowing researchers to conduct high-throughput screening and functional analysis of potential NPY2R-targeting compounds. The kit provides a sensitive and reliable method for evaluating the pharmacological properties of NPY2R drugs, such as agonists and antagonists, in a live-cell environment.
  • SKU: KI001-NPY2R
  • Targeting GPCR: Human Neuropeptide Y Receptor Y2 (NPY2R)
  • GPCR Type: Gi-GPCR
  • Kit components: AAV-CNG/AAV-NPY2R and ACTOne MP Dye
  • Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
  • Shipping: Dry ice.
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SKU: KI001-NPY2R Categories: , ,
The AAV ACTOne GPCR Assay Kits are live-cell-based cAMP biosensor systems designed for rapid and high-throughput functional screening of G-protein coupled receptors (GPCRs) by combination AAV. Kits are available for both Gi-GPCR and Gs-GPCR assays.

AAV ACTOne Gi-GPCR Assay Kits:

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AAV ACTOne Gs-GPCR Assay Kits:

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Advantages of AAV ACTOne GPCR Assay Kits:

  • Combined ACTOne and AAV Technologies
  • Transient Expression of CNG and GPCR
  • Real Time Assay on Living Cell
  • High Sensitivity and Big assay window
  • Both Kinetic and Endpoint Assay
  • Easy to use by mixing the AAVs with cells
  • Faster setup and time efficiency
  • Reduced risk of genetic drift, 
  • Simplified workflows, 
  • flexibility in dynamic control of expression 
  • Low Cost: $498 for one plate
AAV ACTOne GPCR Assay Kit
AAV ACTOne GPCR Assay Kit

ACTOne GPCR Assay

ACTOne™ is the only high-throughput GPCR screening technology that can directly measure the intracellular changes of the secondary messenger cyclic AMP (cAMP) in living cells, in real-time. It uses a proprietary modified cyclic nucleotide-gated (CNG) channel, which is co-localized with adenylate cyclase at the plasma membrane, as a biosensor of cAMP activity. The CNG channel opens when the cAMP level near the plasma membrane increases, resulting in ion flux and cell membrane depolarization. The influx of cations through the CNG channel can be quantified using fluorescent ion indicators or membrane potential (MP) dyes. It provides information on real time intracellular cAMP changes and is highly sensitive. By combining kinetic and endpoint readouts, we are able to capture and analyze transient responses from endogenous GPCRs and weak responses caused by weak Gs or Gi coupled GPCR activities. Using ACTOne, we are able to detect the subcellular cAMP concentration changes directly caused by GPCR activation. Real-time kinetic readouts minimize artifacts, and provide greater content and more statistically relevant data. The intensity of signal increase caused by GPCR activation is directly related to the receptor number on cell surface. Using ACTOne assay, we were able to detect activities of some endogenous Gs coupled receptors in HEK293 cells that have not been reported in literature. In addition, we have also detected weak Gs coupled activity of a GPCR that was widely considered to be only linked to Gq coupled pathway.  The ACTOne assay also provides a useful tool for GPCR de-orphanization.

ACTOne GPCR Assay
ACTOne GPCR Assay
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