pAAVone-AAVs-Pro-CcdB

The pAAVone-AAVs-Pro-CcdB plasmids serves as  destination vectors for efficient ORF cloning into the AAVone system using Gateway technology. This system is part of AAVnerGene’s AAVone platform, which integrates the AAV vector plasmid (pAAVtri) and the Ad helper plasmid (mini-pHelper) and AAV heper plasmid (pRCap) into a single plasmid. The CcdB gene in the plasmid encodes a toxic protein that disrupts DNA gyrase, a bacterial enzyme essential for DNA replication, leading to cell death. This ensures that only bacterial cells with successful recombination events—those replacing the CcdB gene—survive, facilitating positive selection during cloning. By using the pAAVone-AAVs-Pro-CcdB destination plasmids along with the pENTR-GOI vectors from the ORFeome collections, researchers can efficiently generate pAAVone-AAVs-Pro-GOI plasmids. These plasmids can then be used for AAV packaging with the AAVone system, enabling the