Neurotransmitters and neuromodulators Neurotransmitters and neuromodulators are chemical messengers that play essential roles in signal transmission within neural circuits. Neurotransmitters are released from the axon terminals of presynaptic neurons and rapidly excite (e.g., glutamate) or inhibit (e.g., γ-aminobutyric acid, GABA)…
By utilizing different combinations, it is possible to create three distinct types of dual-plasmid systems for AAV production: While all dual-plasmid systems offer specific advantages, only Version V1, as demonstrated by AAVnerGene’s AAVdual system, strikes the optimal balance between packaging…
In the past two decades, adeno-associated virus (AAV) has become one of the most powerful tools (AAV tools) in neuroscience, including for the delivery of AAV biosensors. Its ability to introduce genes into neurons with high specificity and minimal toxicity…
Introduction This protocol quantifies AAV genomic titer using ddPCR. The assay is optimized for BIO-RAD QX200 with an automated droplet generator. Last Modified: May 13, 2024 Equipment BIO-RAD automated droplet generator BIO-RAD QX 200 reader BIO-RAD incubator Reagents DNase I…
Introduction The AAV titration protocol can be used to determine the number of genome-containing particles in an AAV products with qPCR method (Sybr Green). This protocol is suitable for 96/384-well plates with a reaction volume of 10 µL. Equipment Applied…
We are excited to announce that our first AAVone paper has been accepted for publication in MTNA(Molecular Therapy – Nucleic Acids)!The AAVone system consolidates the adenoviral helper genes, AAV packaging genes, and the vector transgene cassette into a single, compact…
We are excited to announce that our scientist, Dr. Bing Wang, will be attending the Association for Research in Vision and Ophthalmology (ARVO), taking place May 4–8 in hashtag#SaltLakeCity, Utah. During the conference, we will present our latest discovery on…
I’m thrilled to share the first results from our large-scale AAV production using the AAVone system in the XDR200 bioreactor. Utilizing the pAAVone-AAV9-CMV-EGFP plasmid, we achieved approximately 1e12 VG/L of AAV9 in VPC2.0 cells—even under non-optimized conditions. Notably, the full…
