AAV Enhancer Library
AAV Enhancer Library
The AAV Enhancer Library is a comprehensive collection of diverse enhancer sequences cloned into AAV vectors, each tagged with unique DNA barcodes. This design enables simultaneous, high-throughput functional screening of numerous enhancer candidates within biological systems. By leveraging our Barcode-Seq technology, researchers can quantitatively assess enhancer activity across different cell types, tissues, or experimental conditions.
Key Features
Broad Diversity: Includes natural and synthetic enhancer elements covering a wide range of regulatory functions.
High-Throughput Screening: Barcode sequencing allows rapid, parallel analysis of enhancer activities in complex samples.
Customizable: Tailor the library composition based on specific research goals or therapeutic targets.
Optimized Vector Systems: Enhancers are cloned into AAV backbones optimized for robust packaging and efficient delivery.
Quantitative and Reliable: Standardized protocols ensure reproducible data and confident selection of functional enhancers.
Applications
Discovery of tissue- or cell-specific enhancers for targeted gene expression.
Fine-tuning transgene expression levels for improved therapeutic outcomes.
Development of inducible or context-dependent enhancer systems.
Supporting basic research, preclinical studies, and gene therapy vector design.
Design of AAV Enhancer Library
The AAV Enhancer Library is thoughtfully designed to enable high-throughput screening of enhancer elements for optimal gene regulation. Each library vector contains the following key components:
Basic Promoter: A minimal promoter that provides a basal level of transcription to evaluate enhancer activity accurately.
Enhancer Elements: Diverse candidate enhancer sequences cloned upstream or downstream of the promoter. These elements are the primary variables tested for their ability to boost transcription.
Reporter Gene: A reporter such as EGFP or luciferase, allowing quantitative measurement of promoter-enhancer activity.
Unique DNA Barcode: Each enhancer variant is linked to a distinct DNA barcode sequence, enabling identification and quantification through next-generation sequencing (Barcode-Seq).
This design allows simultaneous functional assessment of hundreds to thousands of enhancer candidates within complex biological samples, facilitating rapid discovery of effective regulatory elements.
AAV Enhancer Library Cloning
At AAVnerGene, we provide expert AAV Enhancer Library construction services to support your gene therapy and research projects with precision and efficiency. Unlike the AAV Promoter Library, enhancers are typically smaller DNA elements and can often be synthesized directly. Pricing depends on the length and number of enhancers included. We also offer oligo pool synthesis services to streamline library construction.
Oligo Pools Pricing at AAVnerGene
Complexity | 20 – 120nt | 121 – 150nt | 151 – 200nt | 201 – 250nt | 251 – 300nt |
| 2-100 Oligos | $400.00 | $480.00 | $540.00 | $700.00 | $1,000.00 |
| 101-500 Oligos | $800.00 | $960.00 | $1,080.00 | $1,400.00 | $2,000.00 |
| 501-1,000 Oligos | $1,200.00 | $1,440.00 | $1,620.00 | $2,100.00 | $3,000.00 |
| 1,001-2,000 Oligos | $1,600.00 | $1,920.00 | $2,160.00 | $2,800.00 | $4,000.00 |
| 2,001-6,000 Oligos | $2,400.00 | $2,880.00 | $3,240.00 | $4,200.00 | $6,000.00 |
| 6,001-12,000 Oligos | $3,200.00 | $3,840.00 | $4,320.00 | $5,600.00 | $8,000.00 |
| 12,001-18,000 Oligos | $4,000.00 | $4,960.00 | $5,400.00 | $6,800.00 | $10,000.00 |
| 18,001-24,000 Oligos | $5,200.00 | $6,400.00 | $7,200.00 | $8,800.00 | $13,000.00 |
| 24,001-30,000 Oligos | $6,800.00 | $8,400.00 | $9,000.00 | $11,800.00 | $16,400.00 |
| 30,001-42,000 Oligos | $7,800.00 | $9,600.00 | $11,600.00 | $13,000.00 | $20,000.00 |
| 42,001-54,000 Oligos | $9,400.00 | $11,800.00 | $13,200.00 | $16,000.00 | $23,000.00 |
| 54,001-72,000 Oligos | $10,800.00 | $13,000.00 | $14,200.00 | $18,000.00 | $27,000.00 |
| 72,001-96,000 Oligos | $11,800.00 | $14,000.00 | $15,000.00 | $19,000.00 | $29,000.00 |
| 96,001-120,000 Oligos | $12,800.00 | $15,000.00 | $16,000.00 | $20,000.00 | $31,000.00 |
| 120,001-150,000 Oligos | $13,800.00 | $16,000.00 | $17,800.00 | $22,000.00 | $33,000.00 |
| 150,001-180,000 Oligos | $14,800.00 | $18,000.00 | $19,200.00 | $24,000.00 | $36,000.00 |
| 180,001-210,000 Oligos | $15,800.00 | $19,200.00 | $21,800.00 | $26,000.00 | $39,000.00 |
| 210,001-240,000 Oligos | $17,200.00 | $21,800.00 | $23,200.00 | $28,000.00 | $43,000.00 |
| 240,001-300,000 Oligos | $21,000.00 | $25,500.00 | $27,000.00 | $33,000.00 | $49,000.00 |
| 300,001-360,000 Oligos | $25,200.00 | $30,600.00 | $32,400.00 | $39,000.00 | $59,000.00 |
| 360,001-420,000 Oligos | $29,200.00 | $36,000.00 | $38,000.00 | $46,000.00 | $70,000.00 |
| 420,001-480,000 Oligos | $33,600.00 | $41,000.00 | $43,200.00 | $52,800.00 | $82,000.00 |
| 480,001-600,000 Oligos | $41,200.00 | $50,000.00 | $53,200.00 | $64,800.00 | $100,000.00 |
| 600,001-696,000 Oligos | $48,800.00 | $60,000.00 | $63,000.00 | $78,000.00 | $114,000.00 |
For pools with more than 696,000 sequences please contact us at customer@aavnergene.com for pricing
AAV Enhancer library construction can be done using a variety of methods, including restriction enzyme digestion, ligation-independent cloning, or Gibson assembly. Our pricing is dependent on quantity and transfection conditions. Pricing increases with lower copies per cell.
Library complexity | Price | Turnaround Time |
1.00E+07 | $12,800 | 2-3 weeks |
1.00E+08 | $21,800 | 2-3 weeks |
| 1.00E+09 | $68,000 | 3-4 weeks |
| Higher | Please contact us to discuss | |
Note: Complexity is calculated based on the number of clones obtained after transformation. We also provide NGS (Next-Generation Sequencing) services to verify library diversity, available for an additional fee.
AAV Enhancer Library Production
Packaging of AAV Enhancer Library follows the same process as standard AAV packaging. Please contact us (customer@aavnergene.com) to request a customized quote.
AAV Enhancer Library Validation
AAV Enhancer Library Screening
Multiple rounds of AAV-shRNA library evolution involve sequential cycles of infection, selection, and recovery to enrich for shRNAs that produce desired phenotypic effects—such as enhanced gene knockdown, cell survival, or resistance to a treatment.
Each cycle includes:
Infection/Transduction:
Delivering the pooled AAV-shRNA library to target cells or tissues.Selection Pressure:
Applying a biological or pharmacological pressure that favors cells expressing effective shRNAs (e.g., drug treatment, stress conditions, disease models).Recovery & Amplification:
Collecting surviving or phenotypically altered cells, extracting genomic DNA or RNA, and amplifying the shRNA sequences.Next-Generation Sequencing (NGS):
Quantifying the relative abundance of each shRNA to identify enriched or depleted guides.Library Repackaging:
Using the enriched pool to produce a new AAV-shRNA library for the next round.