At AAVnerGene, we offer specialized AAV Productivity Testing Services designed to help researchers and biotech companies optimize their AAV vector production process. Whether you’re aiming for high-titer AAV vectors for gene therapy or research applications, our productivity testing services ensure you get the most efficient system for your needs.

Why AAV Productivity Testing Matters

Achieving high yields of functional AAV vectors is essential for the success of gene therapy programs and research projects. However, AAV production efficiency can vary greatly depending on the AAV production system, plasmid design, the cell line, transfection reagents, plasmids amount, culture medium, and other experimental conditions. By leveraging our expertise and proprietary AAV production systems, we help you:

• Maximize vector yield and full capsid ratio.
• Identify the best production conditions for your project.
• Reduce time and costs associated with low-yield or suboptimal production.

Key Features of Our AAV Productivity Testing Services

1. Comprehensive System Comparison
   We test AAV productivity using various production systems, including our innovative AAVone, AAVdual, and AAVtri systems, alongside standard AAV production methods as controls. This allows you to:

   • Compare AAV yields across multiple platforms.
   • Evaluate the best system for your specific vector and cell line.
     • Adherent and suspension cell culture
     • HEK 293 or HEK 293T
   • Choose the most efficient and scalable method for your needs.
   • Our AAVone system provides a reliable and consistent platform for evaluating various parameters in AAV production. 

2. Customized Testing Design

   • Every project is unique, and so is our approach. We tailor each AAV productivity testing plan to your specific research goals and requirements, offering:

   • Customizable plasmid ratios and conditions.
   • Selection of optimal cell lines and transfection reagents.
   • Flexible testing parameters for different AAV serotypes or constructs.
   • Full/empty ratio monitor in different process steps.

3. Different Scale-up capability

   • Adherent cells: Scalable options range from plates (96-well, 24-well, 6-well) to larger formats such as culture dishes (10 cm, 15 cm), roller bottles (850 cm²), and cell stacks (5-layer and 10-layer).

   • Suspension cells: Growth formats include plates (6-well), flasks (30–5000 mL), and bioreactors (up to 200L) for large-scale production.

4. Precise Titer Quantification
   Our productivity testing includes comprehensive quantification of crude and/or purified AAV yields:

   • Vector genome (VG) titers using qPCR or digital droplet PCR (ddPCR).
   • Capsid particle (CP) titer using Elisa.
   • Full-to-empty capsid ratios (mass photometry), ensuring high-quality functional vectors.

5. Data-Driven Optimization Recommendations
   After completing the testing, we provide a detailed report with actionable insights. You’ll receive:

   • Productivity results across all tested systems.
   • Recommendations on optimizing your production process for higher yields and better quality.
   • Guidance on scaling up production while maintaining efficiency and consistency.

Why Choose AAVnerGene?

• Proven Expertise: With over 15 years of experience in AAV vector design and production, our team provides unmatched expertise in maximizing AAV yields.
• Innovative Systems: Our proprietary AAVone, AAVdual, and AAVtri systems offer cutting-edge solutions for AAV production, giving you more options for efficiency and scalability.
• End-to-End Support: From initial consultation to testing, optimization, and scale-up, we support you throughout the entire AAV production process.

Application of AAV Productivity Testing

• Compare different AAV production systems to identify the most efficient method for your specific project.
• Test various plasmid designs to optimize AAV vector yields and full particle ratios.
  • Different ITR design 
  • Different P5 and Rep design on Rep-Cap expression cassette
  • Function analysis of individual gene on AAV productivity. 
• Optimize plasmid ratios to ensure the highest possible yield while maintaining vector quality.
• Evaluate different cell lines to select the best one for maximizing AAV productivity.
• Compare transfection reagents to find the most effective and scalable solution for your production needs. Positive controls include PEImax, AAVmax, FactorVIR, et al.
• Evaluate different culture media to enhance cell growth and productivity, ensuring the best conditions for AAV production.
• Optimize other experimental conditions to consistently achieve high-quality, reproducible AAV vector production.

Get Started with AAV Productivity Testing

Ready to optimize your AAV production process? Contact AAVnerGene today to discuss your project, and we’ll help you design the best productivity testing plan for your needs.